SMARCD1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88719

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SMARCD1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MGPAPGQGLYRSPMPGAAYPRPGMLPGSRMTPQGPSMGPPGYGGNPSVRPGLAQSGMDQSRKRPAPQQIQQVQQQAVQNRNHNAKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SMARCD1 Antibody - BSA Free

Western Blot: SMARCD1 Antibody [NBP1-88719]

Western Blot: SMARCD1 Antibody [NBP1-88719]

Western Blot: SMARCD1 Antibody [NBP1-88719] - Analysis in human cell line CACO-2.
Immunocytochemistry/ Immunofluorescence: SMARCD1 Antibody [NBP1-88719]

Immunocytochemistry/ Immunofluorescence: SMARCD1 Antibody [NBP1-88719]

Immunocytochemistry/Immunofluorescence: SMARCD1 Antibody [NBP1-88719] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: SMARCD1 Antibody [NBP1-88719]

Immunohistochemistry-Paraffin: SMARCD1 Antibody [NBP1-88719]

Immunohistochemistry-Paraffin: SMARCD1 Antibody [NBP1-88719] - Staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.
Western Blot: SMARCD1 Antibody [NBP1-88719]

Western Blot: SMARCD1 Antibody [NBP1-88719]

Western Blot: SMARCD1 Antibody [NBP1-88719] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
SMARCD1 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: SMARCD1 Antibody - BSA Free [NBP1-88719]

Chromatin Immunoprecipitation-exo-Seq: SMARCD1 Antibody - BSA Free [NBP1-88719]

ChIP-Exo-Seq composite graph for Anti-SMARCD1 (NBP1-88719) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for SMARCD1 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SMARCD1

SMARCD1 is encoded by this gene is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and which are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI and has sequence similarity to the yeast Swp73 protein. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

BAF60Amammalian chromatin remodeling complex BRG1-associated factor 60A, BRG1-associated factor 60A, chromatin remodeling complex BAF60A subunit, CRACD1, Rsc6p, subfamily d, member 1, SWI/SNF complex 60 kDa subunit, SWI/SNF complex 60 kDa subunit A, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily D member 1,60 kDa BRG-1/Brm-associated factor subunit A, Swp73-like protein

Gene Symbol

SMARCD1

Additional SMARCD1 Products

Product Documents for SMARCD1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SMARCD1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SMARCD1 Antibody - BSA Free

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Protocols

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FAQs

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