SMPD1 Antibody (OTI3H7) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-74240
![Western Blot: SMPD1 Antibody (OTI3H7)Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Western-Blot-NBP2-74240-img0001.jpg "Western Blot: SMPD1 Antibody (OTI3H7)Azide and BSA Free [NBP2-74240]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3H7
Format
Azide and BSA Free
Loading...
Product Specifications
Immunogen
Human recombinant protein fragment corresponding to amino acids 62-341 of human SMPD1 (NP_000534) produced in E.coli.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for SMPD1 Antibody (OTI3H7) - Azide and BSA Free
Western Blot: SMPD1 Antibody (OTI3H7)Azide and BSA Free [NBP2-74240]
Western Blot: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SMPD1.![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0021.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0002.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0003.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0004.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0005.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0006.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0007.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0008.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0009.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0010.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0011.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human bladder tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0012.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0013.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0014.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0015.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0016.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0017.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0018.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0019.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human Ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0020.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)![Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]](https://resources.rndsystems.com/images/products/SMPD1-Antibody-OTI3H7-Azide-and-BSA-Free-Immunohistochemistry-NBP2-74240-img0022.jpg "Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]")
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240]
Immunohistochemistry: SMPD1 Antibody (OTI3H7) - Azide and BSA Free [NBP2-74240] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)Applications for SMPD1 Antibody (OTI3H7) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry
1:150
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: SMPD1
Long Name
Sphingomyelin Phosphodiesterase 1, Acid Lysosomal
Alternate Names
aSMase, NPD
Gene Symbol
SMPD1
Additional SMPD1 Products
Product Documents for SMPD1 Antibody (OTI3H7) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SMPD1 Antibody (OTI3H7) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for SMPD1 Antibody (OTI3H7) - Azide and BSA Free
There are currently no reviews for this product. Be the first to review SMPD1 Antibody (OTI3H7) - Azide and BSA Free and earn rewards!
Have you used SMPD1 Antibody (OTI3H7) - Azide and BSA Free?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...