SNF5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-90014

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SNF5 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SVTLLKASEVEEILDGNDEKYKAVSISTEPPTYLREQKAKRNSQWVPTLPNSSHHLDAVPCSTTINRNRMGRDKKRTFPLCFDDHDPAVIHENASQPE

Reactivity Notes

Reactivity reported in scientific literature (PMID: 24335282)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SNF5 Antibody - BSA Free

SNF5 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: SNF5 Antibody - BSA Free [NBP1-90014]

Immunocytochemistry/ Immunofluorescence: SNF5 Antibody - BSA Free [NBP1-90014]

Staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
SNF5 Antibody - BSA Free Immunohistochemistry-Paraffin: SNF5 Antibody - BSA Free [NBP1-90014]

Immunohistochemistry-Paraffin: SNF5 Antibody - BSA Free [NBP1-90014]

Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
SNF5 Antibody - BSA Free Immunohistochemistry-Paraffin: SNF5 Antibody - BSA Free [NBP1-90014]

Immunohistochemistry-Paraffin: SNF5 Antibody - BSA Free [NBP1-90014]

Staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
SNF5 Antibody - BSA Free Immunohistochemistry-Paraffin: SNF5 Antibody - BSA Free [NBP1-90014]

Immunohistochemistry-Paraffin: SNF5 Antibody - BSA Free [NBP1-90014]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.
SNF5 Antibody - BSA Free Immunohistochemistry-Paraffin: SNF5 Antibody - BSA Free [NBP1-90014]

Immunohistochemistry-Paraffin: SNF5 Antibody - BSA Free [NBP1-90014]

Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
SNF5 Antibody - BSA Free Western Blot: SNF5 Antibody - BSA Free [NBP1-90014]

Western Blot: SNF5 Antibody - BSA Free [NBP1-90014]

Analysis in human cell line HEK 293.
Western Blot: SNF5 Antibody [NBP1-90014]

Western Blot: SNF5 Antibody [NBP1-90014]

Western Blot: SNF5 Antibody [NBP1-90014] - Analysis using Anti-SMARCB1 antibody NBP1-90014 (A) shows similar pattern to independent antibody NBP1-90013 (B).
SNF5 Antibody - BSA Free Western Blot: SNF5 Antibody - BSA Free [NBP1-90014]

Western Blot: SNF5 Antibody - BSA Free [NBP1-90014]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Applications for SNF5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SNF5

SNF5 is encoded by this gene is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor, and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.

Alternate Names

BAF47, BAF47hSNF5, BRG1-associated factor 47, hSNFS, Ini1, INI1RTPS1, Integrase interactor 1 protein, malignant rhabdoid tumor suppressor, RDTSNF5 homolog, Sfh1p, SNF5, SNF5L1, Snr1, subfamily b, member 1, sucrose nonfermenting, yeast, homolog-like 1, SWI/SNF related, matrix associated, actin dependent regulator of chromatin, SWI/SNF-related matrix-associated actin-dependent regulator of chromatinsubfamily B member 1

Gene Symbol

SMARCB1

Additional SNF5 Products

Product Documents for SNF5 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SNF5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SNF5 Antibody - BSA Free

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Protocols

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