SNX1 Antibody (6H1) - Azide and BSA Free

Novus Biologicals | Catalog # H00006642-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6H1

Format

Azide and BSA Free
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Product Specifications

Immunogen

SNX1 (NP_003090, 166 a.a. ~ 275 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KVTTQTSLPLFRSKQFAVKRRFSDFLGLYEKLSEKHSQNGFIVPPPPEKSLIGMTKVKVGKEDSSSAEFLEKRRAALERYLQRIVNHPTMLQDPDVREFLEKEELPRAVG

Specificity

SNX1 - sorting nexin 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for SNX1 Antibody (6H1) - Azide and BSA Free

Western Blot: SNX1 Antibody (6H1) [H00006642-M01]

Western Blot: SNX1 Antibody (6H1) [H00006642-M01]

Western Blot: SNX1 Antibody (6H1) [H00006642-M01] - SNX1 monoclonal antibody (M01), clone 6H1 Analysis of SNX1 expression in HeLa.
Immunohistochemistry-Paraffin: SNX1 Antibody (6H1) [H00006642-M01]

Immunohistochemistry-Paraffin: SNX1 Antibody (6H1) [H00006642-M01]

Immunohistochemistry-Paraffin: SNX1 Antibody (6H1) [H00006642-M01] - Analysis of monoclonal antibody to SNX1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
Western Blot: SNX1 Antibody (6H1) [H00006642-M01]

Western Blot: SNX1 Antibody (6H1) [H00006642-M01]

Western Blot: SNX1 Antibody (6H1) [H00006642-M01] - Analysis of SNX1 over-expressed 293 cell line, cotransfected with SNX1 Validated Chimera RNAi ( Cat # H00006642-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SNX1 monoclonal antibody (M01), clone 6H1 (Cat # H00006642-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Western Blot: SNX1 Antibody (6H1) [H00006642-M01]

Western Blot: SNX1 Antibody (6H1) [H00006642-M01]

Western Blot: SNX1 Antibody (6H1) [H00006642-M01] - Analysis of SNX1 expression in transfected 293T cell line by SNX1 monoclonal antibody (M01), clone 6H1.Lane 1: SNX1 transfected lysate(59.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: SNX1 Antibody (6H1) [H00006642-M01] - Detection limit for recombinant GST tagged SNX1 is approximately 0.03ng/ml as a capture antibody.

Applications for SNX1 Antibody (6H1) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SNX1

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This endosomal protein regulates the cell-surface expression of epidermal growth factor receptor. This protein also has a role in sorting protease-activated receptor-1 from early endosomes to lysosomes. This protein may form oligomeric complexes with family members. This gene results in three transcript variants encoding distinct isoforms.

Alternate Names

HsT17379, MGC8664, SNX1Asorting nexin 1A, sorting nexin 1, sorting nexin-1, Vps5

Entrez Gene IDs

6642 (Human)

Gene Symbol

SNX1

OMIM

601 (Human)

Additional SNX1 Products

Product Documents for SNX1 Antibody (6H1) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SNX1 Antibody (6H1) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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