SNX15 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85866

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SNX15 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EEDGEGGPTPAYLSQATELITQALRDEKAGAYAAALQGYRDGVHVLLQGVPSDPLPARQEGVKKKAAEYLKRAEEILRLHLSQLPP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SNX15 Antibody - BSA Free

Western Blot: SNX15 Antibody [NBP1-85866]

Western Blot: SNX15 Antibody [NBP1-85866]

Western Blot: SNX15 Antibody [NBP1-85866] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: SNX15 Antibody [NBP1-85866]

Immunocytochemistry/ Immunofluorescence: SNX15 Antibody [NBP1-85866]

Immunocytochemistry/Immunofluorescence: SNX15 Antibody [NBP1-85866] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & cytosol.
Immunohistochemistry-Paraffin: SNX15 Antibody [NBP1-85866]

Immunohistochemistry-Paraffin: SNX15 Antibody [NBP1-85866]

Immunohistochemistry-Paraffin: SNX15 Antibody [NBP1-85866] - Staining of human kidney shows moderate cytoplasmic and nucleolar positivity in tubule cells.

Applications for SNX15 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SNX15

SNX15 encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. Overexpression of this gene results in a decrease in the processing of insulin and hepatocyte growth factor receptors to their mature subunits. This decrease is caused by the mislocalization of furin, the endoprotease responsible for cleavage of insulin and hepatocyte growth factor receptors. This protein is involved in endosomal trafficking from the plasma membrane to recycling endosomes or the trans-Golgi network. This gene encodes two transcript variants encoding distinct isoforms.

Alternate Names

clone iota unknown protein, HSAF001435, sorting nexin 15, sorting nexin-15

Gene Symbol

SNX15

Additional SNX15 Products

Product Documents for SNX15 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SNX15 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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