SOAT1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-32052

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOAT1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: MVGEEKMSLRNRLSKSRENPEEDEDQRNPAKESLETPSNGRIDIKQLIAKKIKLTAEAEELKPF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOAT1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SOAT1 Antibody [NBP2-32052]

Immunocytochemistry/ Immunofluorescence: SOAT1 Antibody [NBP2-32052]

Immunocytochemistry/Immunofluorescence: ACAT Antibody [NBP2-32052] - Immunofluorescent staining of human cell line A-431 shows localization to endoplasmic reticulum.
Immunohistochemistry-Paraffin: SOAT1 Antibody [NBP2-32052]

Immunohistochemistry-Paraffin: SOAT1 Antibody [NBP2-32052]

Immunohistochemistry-Paraffin: ACAT Antibody [NBP2-32052] - Staining in human adrenal gland and cerebral cortex tissues using anti-SOAT1 antibody. Corresponding SOAT1 RNA-seq data are presented for the same tissues.
Immunohistochemistry: SOAT1 Antibody [NBP2-32052]

Immunohistochemistry: SOAT1 Antibody [NBP2-32052]

Immunohistochemistry: ACAT Antibody [NBP2-32052] - Staining of liver.
Immunohistochemistry: SOAT1 Antibody [NBP2-32052]

Immunohistochemistry: SOAT1 Antibody [NBP2-32052]

Immunohistochemistry: ACAT Antibody [NBP2-32052] - Staining of liver cancer.
Immunohistochemistry-Paraffin: SOAT1 Antibody [NBP2-32052]

Immunohistochemistry-Paraffin: SOAT1 Antibody [NBP2-32052]

Immunohistochemistry-Paraffin: ACAT Antibody [NBP2-32052] - Staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
Immunohistochemistry-Paraffin: SOAT1 Antibody [NBP2-32052]

Immunohistochemistry-Paraffin: SOAT1 Antibody [NBP2-32052]

Immunohistochemistry-Paraffin: ACAT Antibody [NBP2-32052] - Staining of human cerebral cortex shows low expression as expected.

Applications for SOAT1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOAT1

Acely-CoA:cholestorl acyltransferase (ACAT) is an integral membrane enzyme that belongs to type-B carboxylesterase/lipase family. ACAT catalyzes the formation of cholesteryl esters from cholesterol and long-chain fatty-acyl-coenzyme A (1). ACAT also function as a regulator of intracellular cholesterol homeostasis at single cell level. Two isoform of ACAT exists, ACAT1 and ACAT2, are expressed in liver and intestine. However, ACAT1 is only found in macrophages and SMCs (2). The accumulation of cholesteryl esters produced by ACAT in macrophages can lead to foam cell formation, an indication of early stage atherosclerosis. ACAT activity has been linked to Alzehimer's Diease, condition caused by excess amount of amyloid beta-peptide (Abeta). Atherogenic lipoproteins activate ACAT activity, where active ACAT can modulate the level of Abeta (3).

Long Name

Sterol O-Acyltransferase 1

Alternate Names

ACACT, ACAT-1, EC 2.3.1.26, SOAT1

Gene Symbol

SOAT1

UniProt

P35610 (Human)

Additional SOAT1 Products

Product Documents for SOAT1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SOAT1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Obesity

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Protocols

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FAQs

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