SOD2/Mn-SOD Antibody (8H3D2) - BSA Free

Novus Biologicals | Catalog # NBP2-61883

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 8H3D2

Format

BSA Free
View all SOD2/Mn-SOD Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human SOD2/Mn-SOD (AA: 1-222) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

24.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOD2/Mn-SOD Antibody (8H3D2) - BSA Free

Western Blot: SOD2/Mn-SOD Antibody (8H3D2)BSA Free [NBP2-61883]

Western Blot: SOD2/Mn-SOD Antibody (8H3D2)BSA Free [NBP2-61883]

Western Blot: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis using SOD2 mAb against human SOD2 (AA: 1-222) recombinant protein. (Expected MW is 50.7 kDa)
Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody (8H3D2) - BSA Free [NBP2-61883]

Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody (8H3D2) - BSA Free [NBP2-61883]

Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis of Hela cells using SOD2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3D2) - BSA Free [NBP2-61883]

Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3D2) - BSA Free [NBP2-61883]

Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis of paraffin-embedded rectum cancer tissues using SOD2 mouse mAb with DAB staining.
Flow Cytometry: SOD2/Mn-SOD Antibody (8H3D2) - BSA Free [NBP2-61883]

Flow Cytometry: SOD2/Mn-SOD Antibody (8H3D2) - BSA Free [NBP2-61883]

Flow Cytometry: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis of Hela cells using SOD2 mouse mAb (green) and negative control (red).
Western Blot: SOD2/Mn-SOD Antibody (8H3D2)BSA Free [NBP2-61883]

Western Blot: SOD2/Mn-SOD Antibody (8H3D2)BSA Free [NBP2-61883]

Western Blot: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis using SOD2 mAb against HEK293 (1) and SOD2 (AA: 1-222)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3D2) - BSA Free [NBP2-61883]

Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3D2) - BSA Free [NBP2-61883]

Immunohistochemistry: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Analysis of paraffin-embedded ovarian cancer tissues using SOD2 mouse mAb with DAB staining.
ELISA: SOD2/Mn-SOD Antibody (8H3D2) - BSA Free [NBP2-61883]

ELISA: SOD2/Mn-SOD Antibody (8H3D2) - BSA Free [NBP2-61883]

ELISA: SOD2/Mn-SOD Antibody (8H3D2) [NBP2-61883] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Applications for SOD2/Mn-SOD Antibody (8H3D2) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:50-1:250

Immunohistochemistry

1:200-1:1000

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOD2/Mn-SOD

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.;

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Gene Symbol

SOD2

Additional SOD2/Mn-SOD Products

Product Documents for SOD2/Mn-SOD Antibody (8H3D2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SOD2/Mn-SOD Antibody (8H3D2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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