SOD2/Mn-SOD Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-20535

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Alligator, Avian

Cited:

Human, Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOD2/Mn-SOD Primary Antibodies »

Product Specifications

Immunogen

Full length human SOD2/Mn-SOD Recombinant protein.

Localization

Mitochondrion matrix

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SOD2/Mn-SOD Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535]

Immunocytochemistry/ Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535]

Immunocytochemistry/Immunofluorescence: SOD2/Mn-SOD Antibody [NBP2-20535] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535]

Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535]

Immunohistochemistry-Paraffin: SOD2/Mn-SOD Antibody [NBP2-20535] - Human breast carcinoma. SOD2 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
SOD2/Mn-SOD Antibody

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] -

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
SOD2/Mn-SOD Antibody - BSA Free

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] -

SOD2 antibody detects SOD2 protein by western blot analysis. Mouse tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (NBP2-20535) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SOD2/Mn-SOD Antibody - BSA Free

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] -

SOD2 antibody detects SOD2 protein by western blot analysis. Rat tissue extracts (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (NBP2-20535) diluted by 1:2500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
SOD2/Mn-SOD Antibody - BSA Free

Western Blot: SOD2/Mn-SOD Antibody [NBP2-20535] -

Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD2 antibody (NBP2-20535) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for SOD2/Mn-SOD Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Assay dependent

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000

Reviewed Applications

Read 1 review rated 5 using NBP2-20535 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SOD2/Mn-SOD

This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Long Name

Superoxide Dismutase-2

Alternate Names

IPO-B, Mn SOD, MnSOD, SOD, Mitochodrial

Gene Symbol

SOD2

Additional SOD2/Mn-SOD Products

Product Documents for SOD2/Mn-SOD Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for SOD2/Mn-SOD Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

Citations for SOD2/Mn-SOD Antibody - BSA Free

Customer Reviews for SOD2/Mn-SOD Antibody - BSA Free (1)

5 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: mouse liver lysate
    Species: Mouse
    Verified Customer | Posted 05/06/2013
    SOD2/Mn-SOD Antibody - BSA Free NBP2-20535

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Protocols

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