Sodium Potassium ATPase Alpha 1 Antibody (HL114) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13660

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL114 expressed in HEK293

Format

Azide and BSA Free
View all Sodium Potassium ATPase Alpha 1 Primary Antibodies »

Product Specifications

Immunogen

The immunogen used to generate this antibody corresponds to chicken Sodium Potassium ATPase Alpha 1.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for Sodium Potassium ATPase Alpha 1 Antibody (HL114) - Azide and BSA Free

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]

Immunocytochemistry/ Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]

Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660]

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Sodium Potassium ATPase Alpha 1 Antibody (HL114)

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] -

Immunohistochemistry-Paraffin: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Sodium Potassium ATPase Alpha 1 antibody [HL114] detects Sodium Potassium ATPase Alpha 1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Sodium Potassium ATPase Alpha 1 stained by Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Sodium Potassium ATPase Alpha 1 Antibody (HL114)

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] -

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] - Drosophila tissue extract (5 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium Potassium ATPase Alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Sodium Potassium ATPase Alpha 1 Antibody (HL114) - Azide and BSA Free

Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (HL114) [NBP3-13660] -

A431 whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Sodium/Potassium ATPase alpha 1 antibody [HL114] (NBP3-13660) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Sodium Potassium ATPase Alpha 1 Antibody (HL114) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sodium Potassium ATPase Alpha 1

Sodium Potassium ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The Sodium Potassium ATPase enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide (Sodium Potassium ATPase Alpha 1) has been shown to be the catalytically active subunit, whereas the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Sodium Potassium ATPase alpha-1 subunit, in the brain, is expressed in both neuronal and non-neuronal cells.

Long Name

Sodium/potassium-transporting ATPase subunit alpha-1

Alternate Names

ATP1A1, EC 7.2.2.13

Gene Symbol

ATP1A1

Additional Sodium Potassium ATPase Alpha 1 Products

Product Documents for Sodium Potassium ATPase Alpha 1 Antibody (HL114) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Sodium Potassium ATPase Alpha 1 Antibody (HL114) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Sodium Potassium ATPase Alpha 1 Antibody (HL114) - Azide and BSA Free

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  • Q: I am looking forward to pick up a plasma membrane marker. I have gastric cancer cell line and I am preparing PM out of that. To just check the quality of prep, I need a plasma membrane marker.

    A:

    Our sodium potassium ATPase and plasma membrane products may be of use to you. Please contact us at technical@novusbio.com with any additional questions.

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