Sortilin Antibody (CL6526) - BSA Free

Novus Biologicals | Catalog # NBP2-76498

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL6526

Format

BSA Free
View all Sortilin Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q99523, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Sortilin Antibody (CL6526) - BSA Free

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Analysis in human cerebral cortex and lymph node tissues. Corresponding SORT1 RNA-seq data are presented for the same tissues.
Western Blot: Sortilin Antibody (CL6526) [NBP2-76498]

Western Blot: Sortilin Antibody (CL6526) [NBP2-76498]

Western Blot: Sortilin Antibody (CL6526) [NBP2-76498] - Analysis in Rh30 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SORT1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: Sortilin Antibody (CL6526) [NBP2-76498]

Immunocytochemistry/ Immunofluorescence: Sortilin Antibody (CL6526) [NBP2-76498]

Immunocytochemistry/Immunofluorescence: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of RH-30 cells using the Anti-SORT1 monoclonal antibody, showing specific staining of the golgi apparatus in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Western Blot: Sortilin Antibody (CL6526) [NBP2-76498]

Western Blot: Sortilin Antibody (CL6526) [NBP2-76498]

Western Blot: Sortilin Antibody (CL6526) [NBP2-76498] - Analysis in human cell line SK-MEL-30.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of human lymph node shows only weak positivity in a subset of lymphoid cells, as expected.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of mouse cerebral cortex shows strong cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498]

Immunohistochemistry-Paraffin: Sortilin Antibody (CL6526) [NBP2-76498] - Staining of mouse hippocampus shows strong cytoplasmic positivity in both pyramidal neurons and granular cells.

Applications for Sortilin Antibody (CL6526) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Sortilin

Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.

Alternate Names

Gp95, Ntr3, SORT1

Gene Symbol

SORT1

Additional Sortilin Products

Product Documents for Sortilin Antibody (CL6526) - BSA Free

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Product Specific Notices for Sortilin Antibody (CL6526) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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