SOX11 Antibody (CL0142) - BSA Free

Novus Biologicals | Catalog # NBP2-14782

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL0142

Format

BSA Free
View all SOX11 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P48431, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Expected to cross react based on sequence identity: Rat (82%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for SOX11 Antibody (CL0142) - BSA Free

Western Blot: SOX11 Antibody (CL0142) [NBP2-14782]

Western Blot: SOX11 Antibody (CL0142) [NBP2-14782]

Western Blot: SOX11 Antibody (CL0142) [NBP2-14782] - Analysis in control (vector only transfected HEK293T lysate) and sOX11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: SOX11 Antibody (CL0142) [NBP2-14782]

Immunocytochemistry/ Immunofluorescence: SOX11 Antibody (CL0142) [NBP2-14782]

Immunocytochemistry/Immunofluorescence: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of RH-30 cells using the Anti-SOX11 monoclonal antibody, showing specific staining in the nucleus and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of eye in mouse embryo E14 retina shows moderate nuclear positivity in developing retina.
Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of human chronic lymphoid leukemia shows no positivity in tumor cells.
Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of human mantle cell lymphoma shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782]

Immunohistochemistry-Paraffin: SOX11 Antibody (CL0142) [NBP2-14782] - Staining of mouse embryo E14 shows nuclear immunoreactivity in the developing forebrain neurons.
Simple Western: SOX11 Antibody (CL0142) [NBP2-14782]

Simple Western: SOX11 Antibody (CL0142) [NBP2-14782]

Simple Western: SOX11 Antibody (CL0142) [NBP2-14782] - Simple Western lane view shows a specific band for SOX11 in 0.2 mg/ml of U-251MG sp (left) and SHSY5Y (right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for SOX11 Antibody (CL0142) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Simple Western

5 ug/ml

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp and SHSY5Y, separated by Size, antibody dilution of 5 ug/mL, apparent MW was 53 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX11

SOX11 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. The protein may function in the developing nervous system and play a role in tumorigenesis.

Long Name

Transcription Factor SRY (Sex Determining Region Y)-box 11

Alternate Names

SRY (sex determining region Y)-box 11, SRY (sex-determining region Y)-box 11, SRY-related HMG-box gene 11, transcription factor SOX-11

Gene Symbol

SOX11

Additional SOX11 Products

Product Documents for SOX11 Antibody (CL0142) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SOX11 Antibody (CL0142) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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