SOX11 Antibody (CL0142) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-43690
Key Product Details
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # CL0142
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of P48431, with the exact immunogen sequence remaining proprietary.
Reactivity Notes
Expected to cross react based on sequence identity: Rat (82%). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for SOX11 Antibody (CL0142) - Azide and BSA Free
![SOX11 Antibody (CL0142) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]](https://resources.rndsystems.com/images/products/nbp3-43690_mouse-sox11-mab-cl0142-azide-and-bsa-free-282202512135883.jpg "Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]")
Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]
Staining of eye in mouse embryo E14 retina shows moderate nuclear positivity in developing retina.![SOX11 Antibody (CL0142) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]](https://resources.rndsystems.com/images/products/nbp3-43690_mouse-sox11-mab-cl0142-azide-and-bsa-free-282202512394459.jpg "Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]")
Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]
Staining of human chronic lymphocytic leukemia shows no nuclear positivity in tumor cells as expected.![SOX11 Antibody (CL0142) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]](https://resources.rndsystems.com/images/products/nbp3-43690_mouse-sox11-mab-cl0142-azide-and-bsa-free-282202513135917.jpg "Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]")
Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]
Staining of human mantle cell lymphoma shows moderate to strong nuclear positivity in tumor cells.![SOX11 Antibody (CL0142) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]](https://resources.rndsystems.com/images/products/nbp3-43690_mouse-sox11-mab-cl0142-azide-and-bsa-free-282202513381537.jpg "Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]")
Immunohistochemistry-Paraffin: SOX11 Antibody [NBP3-43690]
Staining of mouse embryo E14 shows strong nuclear positivity in the developing forebrain.![SOX11 Antibody (CL0142) - Azide and BSA Free Western Blot: SOX11 Antibody [NBP3-43690]](https://resources.rndsystems.com/images/products/nbp3-43690_mouse-sox11-mab-cl0142-azide-and-bsa-free-282202513245481.jpg "Western Blot: SOX11 Antibody [NBP3-43690]")
Western Blot: SOX11 Antibody [NBP3-43690]
Analysis in control (vector only transfected HEK293T lysate) and sOX11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).![SOX11 Antibody (CL0142) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: SOX11 Antibody [NBP3-43690]](https://resources.rndsystems.com/images/products/nbp3-43690_mouse-sox11-mab-cl0142-azide-and-bsa-free-28220251324541.jpg "Immunocytochemistry/ Immunofluorescence: SOX11 Antibody [NBP3-43690]")
Immunocytochemistry/ Immunofluorescence: SOX11 Antibody [NBP3-43690]
Staining of RH-30 cells using the Anti-SOX11 monoclonal antibody).Applications for SOX11 Antibody (CL0142) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:500 - 1:1000
Simple Western
1:20
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100
See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp and SHSY5Y, separated by Size, antibody dilution of 1:20, apparent MW was 53 kDa
See Simple Western Antibody Database for Simple Western validation: Tested in U-251MG sp and SHSY5Y, separated by Size, antibody dilution of 1:20, apparent MW was 53 kDa
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at -20C prior to opening. Aliquot reconstituted liquid and and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing.
Calculators
Background: SOX11
Long Name
Transcription Factor SRY (Sex Determining Region Y)-box 11
Alternate Names
SRY (sex determining region Y)-box 11, SRY (sex-determining region Y)-box 11, SRY-related HMG-box gene 11, transcription factor SOX-11
Gene Symbol
SOX11
Additional SOX11 Products
Product Documents for SOX11 Antibody (CL0142) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SOX11 Antibody (CL0142) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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