SOX2 Antibody (2E1) - BSA Free

Name: SOX2 Antibody (2E1) - BSA Free
Brand: Novus Biologicals
SKU: NBP3-26305
Price: 469 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-26305

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Product Documents

Recombinant monoclonal antibody expressed in HEK293F cells

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2E1 expressed in HEK293

Format

BSA Free

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Product Specifications

Immunogen

A synthesized peptide derived from Human SOX2 [UniProt P48431]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit SOX2 Antibody (2E1) - BSA Free (NBP3-26305) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for SOX2 Antibody (2E1) - BSA Free

Immunohistochemistry: SOX2 Antibody (2E1) [NBP3-26305] -

Immunohistochemistry: SOX2 Antibody (2E1) [NBP3-26305] - Image of SOX2 Antibody (2E1) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Western Blot: SOX2 Antibody (2E1) [NBP3-26305] -

Western Blot: SOX2 Antibody (2E1) [NBP3-26305] - Positive Western Blot detected in: U-251 whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate.
All lanes: SOX2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 35 kDa
Observed band size: 40 kDa

Applications for SOX2 Antibody (2E1) - BSA Free

Application

Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: SOX2

The SOX-2 protein is a transcription factor that is critical for early embryogenesis and for embryonic stem cell pluripotency. The SOX-2 protein is also significant for eye development. SOX 2, also known as SRY related HMG BOX gene 2, belongs to the SOX (SRY-box containing gene) gene family. The SOX-2 protein is a transcription factor since it binds to DNA and regulates activity of other genes. All SOX family proteins share HMG box domains for DNA binding.

Long Name

Transcription Factor SOX2

Alternate Names

ANOP3, MCOPS3, MGC2413, SRY (sex determining region Y)-box 2, SRY-related HMG-box gene 2, transcription factor SOX2, transcription factor SOX-2

Gene Symbol

SOX2

Additional SOX2 Products

Product Documents for SOX2 Antibody (2E1) - BSA Free

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Product Specific Notices for SOX2 Antibody (2E1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.