SOX30 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86503

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SOX30 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPSPVTLFQPSVSSAAQVAVQDPSLPVYPALPPQRFTGPSQTDTHQLHSEATHTVKQPTPVSLESANRISSSASTAHARFATSTIQPPREYSSVSPCPRSAPIPQASPIPHP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SOX30 Antibody - BSA Free

Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503]

Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503]

Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503] - Staining of human Skin shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503]

Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503]

Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503] - Staining of human Liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503]

Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503]

Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503]

Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503]

Immunohistochemistry-Paraffin: SOX30 Antibody [NBP1-86503] - Staining of human Testis shows strong nuclear positivity in spermatids.
SOX30 Antibody - BSA Free Immunohistochemistry-Paraffin: SOX30 Antibody - BSA Free [NBP1-86503]

Immunohistochemistry-Paraffin: SOX30 Antibody - BSA Free [NBP1-86503]

Analysis in human testis and skin tissues Corresponding SOX30 RNA-seq data are presented for the same tissues.
SOX30 Antibody - BSA Free Western Blot: SOX30 Antibody - BSA Free [NBP1-86503]

Western Blot: SOX30 Antibody - BSA Free [NBP1-86503]

Analysis in control (vector only transfected HEK293T lysate) and SOX30 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
SOX30 Antibody - BSA Free Western Blot: SOX30 Antibody - BSA Free [NBP1-86503]

Western Blot: SOX30 Antibody - BSA Free [NBP1-86503]

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: Human cell line RT-4
Lane 3: Human cell line U-251MG sp
SOX30 Antibody - BSA Free Chromatin Immunoprecipitation ChIP: SOX30 Antibody - BSA Free

Chromatin Immunoprecipitation ChIP: SOX30 Antibody - BSA Free

ChIP-Exo-Seq composite graph for Anti-SOX30 tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh's Lab at Cornell University.

Applications for SOX30 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SOX30

The SOX30 gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after for

Alternate Names

Sox30 protein type II, SRY (sex determining region Y)-box 30, transcription factor SOX-30

Gene Symbol

SOX30

Additional SOX30 Products

Product Documents for SOX30 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SOX30 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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