SOX6 Antibody (CL5690) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44223

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL5690

Format

Azide and BSA Free
View all SOX6 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P35712, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for SOX6 Antibody (CL5690) - Azide and BSA Free

SOX6 Antibody (CL5690) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: SOX6 Antibody [NBP3-44223]

Immunocytochemistry/ Immunofluorescence: SOX6 Antibody [NBP3-44223]

Staining of CACO-2 cells using the Anti-SOX6 monoclonal antibody).
SOX6 Antibody (CL5690) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Analysis in human small intestine and tonsil tissues using NBP3-44223 antibody. Corresponding SOX6 RNA-seq data are presented for the same tissues.
SOX6 Antibody (CL5690) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Staining of human glioma shows moderate to strong nuclear positivity in tumor cells.
SOX6 Antibody (CL5690) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Staining of human skin shows moderate nuclear positivity in squamous epithelial cells.
SOX6 Antibody (CL5690) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Staining of human small intestine shows moderate nuclear positivity in glandular cells.
SOX6 Antibody (CL5690) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Staining of human testis shows weak to moderate nuclear positivity in a subset of cells in seminiferous ducts.
SOX6 Antibody (CL5690) - Azide and BSA Free Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Immunohistochemistry-Paraffin: SOX6 Antibody [NBP3-44223]

Staining of human tonsil shows no positivity in non-germinal center cells as expected.

Applications for SOX6 Antibody (CL5690) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation Permeabilization: PFA/Triton X-100

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SOX6

Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at a minimum of 40 different loci that rapidly diverged in various animal lineages. At present 30 Sox genes have been identified, and members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal.

Long Name

SRY-related HMG-box 6

Alternate Names

SOXD

Gene Symbol

SOX6

Additional SOX6 Products

Product Documents for SOX6 Antibody (CL5690) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SOX6 Antibody (CL5690) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Chondrogenesis Markers

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Protocols

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FAQs

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