Sp3 Antibody (4E5) - Azide and BSA Free

Novus Biologicals | Catalog # H00006670-M09

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4E5

Format

Azide and BSA Free
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Product Specifications

Immunogen

SP3 (NP_003102, 287 a.a. ~ 430 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. TAGINADGHLINTGQAMDSSDNSERTGERVSPDINETNTDTDLFVPTSSSSQLPVTIDSTGILQQNTNSLTTSSGQVHSSDLQGNYIQSPVSEETQAQNIQVSTAQPVVQHLQLQESQQPTSQAQIVQGITPQTIHGVQASGQN*

Specificity

SP3 (4E5)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Sp3 Antibody (4E5) - Azide and BSA Free

Western Blot: Sp3 Antibody (4E5) [H00006670-M09]

Western Blot: Sp3 Antibody (4E5) [H00006670-M09]

Western Blot: Sp3 Antibody (4E5) [H00006670-M09] - Analysis of SP3 expression in human stomach.
Immunocytochemistry/ Immunofluorescence: Sp3 Antibody (4E5) [H00006670-M09]

Immunocytochemistry/ Immunofluorescence: Sp3 Antibody (4E5) [H00006670-M09]

Immunocytochemistry/Immunofluorescence: Sp3 Antibody (4E5) [H00006670-M09] - Analysis of monoclonal antibody to SP3 on HeLa cell. Antibody concentration 10 ug/ml

Applications for Sp3 Antibody (4E5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against tissue lysate and recombinant protein for WB. It has been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Sp3

This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted.

Long Name

Sp3 Transcription Factor

Alternate Names

SPR-2

Entrez Gene IDs

6670 (Human)

Gene Symbol

SP3

OMIM

601804 (Human)

Additional Sp3 Products

Product Documents for Sp3 Antibody (4E5) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Sp3 Antibody (4E5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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