SPAM1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93736

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SPAM1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 100-300 of human SPAM1 (NP_003108.2). PYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPAM1 Antibody - BSA Free

Western Blot: SPAM1 AntibodyAzide and BSA Free [NBP2-93736]

Western Blot: SPAM1 AntibodyAzide and BSA Free [NBP2-93736]

Western Blot: SPAM1 Antibody [NBP2-93736] - Analysis of extracts of various cell lines, using SPAM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: SPAM1 Antibody - Azide and BSA Free [NBP2-93736]

Immunocytochemistry/ Immunofluorescence: SPAM1 Antibody - Azide and BSA Free [NBP2-93736]

Immunocytochemistry/Immunofluorescence: SPAM1 Antibody [NBP2-93736] - Analysis of human testis cells using SPAM1 Rabbit pAb at dilution of 1:150 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: SPAM1 Antibody - Azide and BSA Free [NBP2-93736]

Immunohistochemistry-Paraffin: SPAM1 Antibody - Azide and BSA Free [NBP2-93736]

Immunohistochemistry-Paraffin: SPAM1 Antibody [NBP2-93736] - Rat testis using SPAM1 Rabbit pAb at dilution of 1:100 (40x lens).
Immunocytochemistry/ Immunofluorescence: SPAM1 Antibody - Azide and BSA Free [NBP2-93736]

Immunocytochemistry/ Immunofluorescence: SPAM1 Antibody - Azide and BSA Free [NBP2-93736]

Immunocytochemistry/Immunofluorescence: SPAM1 Antibody [NBP2-93736] - Analysis of rat testis cells using SPAM1 Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for SPAM1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPAM1

Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple protein isoforms are encoded by transcript variants of this gene. [provided by RefSeq]

Long Name

Sperm Adhesion Molecule 1

Alternate Names

HYA1, HYAL3, HYAL5, PH20, SPAG15

Gene Symbol

SPAM1

Additional SPAM1 Products

Product Documents for SPAM1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SPAM1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SPAM1 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review SPAM1 Antibody - BSA Free and earn rewards!

Have you used SPAM1 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies