SPARC Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-80971

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunohistochemistry-Frozen, Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SPARC Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDY

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (85%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPARC Antibody - BSA Free

Western Blot: SPARC Antibody [NBP1-80971]

Western Blot: SPARC Antibody [NBP1-80971]

Western Blot: SPARC Antibody [NBP1-80971] - Analysis in human cell lines U-251MG and MCF-7. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Western Blot: SPARC Antibody [NBP1-80971]

Western Blot: SPARC Antibody [NBP1-80971]

Western Blot: SPARC Antibody [NBP1-80971] - Analysis using Anti-SPARC antibody NBP1-80971 (A) shows similar pattern to independent antibody NBP1-80972 (B).
Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971]

Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971]

Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human placenta shows moderate cytoplasmic positivity in fibroblasts.
Western Blot: SPARC Antibody [NBP1-80971]

Western Blot: SPARC Antibody [NBP1-80971]

Western Blot: SPARC Antibody [NBP1-80971] - Analysis in control (vector only transfected HEK293T lysate) and SPARC over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971]

Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971]

Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971]

Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971]

Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human endometrium shows moderate cytoplasmic positivity in stromal cells.
Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971]

Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971]

Immunohistochemistry-Paraffin: SPARC Antibody [NBP1-80971] - Staining of human skin shows moderate cytoplasmic positivity in fibroblasts.

Applications for SPARC Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Frozen

1:100-1:2000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in IHC-FR reported in scientific literature (PMID 24552219).

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPARC

Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.

Long Name

Secreted Protein Acidic and Rich in Cysteine

Alternate Names

BM-40, Osteonectin

Entrez Gene IDs

6678 (Human)

Gene Symbol

SPARC

UniProt

P09486

Additional SPARC Products

Product Documents for SPARC Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPARC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SPARC Antibody - BSA Free

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Protocols

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