SPDEF Antibody (4A5) - Azide and BSA Free

Novus Biologicals | Catalog # H00025803-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4A5

Format

Azide and BSA Free
View all SPDEF Primary Antibodies »

Product Specifications

Immunogen

SPDEF (NP_036523, 92 a.a. ~ 200 a.a) partial recombinant protein with GST tag. QCPVIDSQAPAGSLDLVPGGLTLEEHSLEQVQSMVVGEVLKD IETACKLLNITADPMDWSPSNVQKWLLWTEHQYRLPPMGKAF QELAGKELCAMSEEQFRQRSPLGGD

Specificity

SPDEF - SAM pointed domain containing ets transcription factor

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for SPDEF Antibody (4A5) - Azide and BSA Free

Western Blot: SPDEF Antibody (4A5) [H00025803-M01]

Western Blot: SPDEF Antibody (4A5) [H00025803-M01]

Western Blot: SPDEF Antibody (4A5) [H00025803-M01] - Analysis of SPDEF expression in transfected 293T cell line by SPDEF monoclonal antibody (M01), clone 4A5.Lane 1: SPDEF transfected lysate(37.518 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: SPDEF Antibody (4A5) [H00025803-M01] - Detection limit for recombinant GST tagged SPDEF is 0.3 ng/ml as a capture antibody.

Applications for SPDEF Antibody (4A5) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPDEF

SPDEF, also known as PDEF is an ETS transcription factor expressed in the prostate epithelial cells. It is thought to act as an androgen-independent transactivator of the prostate-specific antigen (PSA) promoter and also as a transcriptional activator for the SERPINB5 promoter. It is highly expressed in prostate carcinoma cells and is thought to be involved in the regulation of the prostate gland and/or prostate cancer development. It is also expressed in epithelial breast carcinoma.

Alternate Names

bA375E1.3, PDEFRP11-375E1__A.3, Prostate epithelium-specific Ets transcription factor, Prostate-derived Ets factor, Prostate-specific Ets, PSE, SAM pointed domain containing ets transcription factor, SAM pointed domain-containing Ets transcription factor

Entrez Gene IDs

25803 (Human)

Gene Symbol

SPDEF

OMIM

608144 (Human)

UniProt

NP_036523 (Human)

Additional SPDEF Products

Product Documents for SPDEF Antibody (4A5) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SPDEF Antibody (4A5) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SPDEF Antibody (4A5) - Azide and BSA Free

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Protocols

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FAQs

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