Spectrin beta 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-86088

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Spectrin beta 2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPRIDDIFERSQNIVTDSSSLSAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHRAQQYYFDAAEAEAWMSEQEL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Spectrin beta 2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Spectrin beta 2 Antibody [NBP1-86088]

Immunocytochemistry/ Immunofluorescence: Spectrin beta 2 Antibody [NBP1-86088]

Immunocytochemistry/Immunofluorescence: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Spectrin beta 2 Antibody - BSA Free Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody - BSA Free [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody - BSA Free [NBP1-86088]

Immunohistochemistry analysis in human lung and skeletal muscle tissues using HPA012685 antibody. Corresponding SPTBN1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human lung shows weak to moderate positivity in respiratory epithelial cells.
Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - staining of human cerebral cortex shows moderate to strong positivity in neuropil.
Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human kidney shows weak to moderate membranous positivity in cells in tubules.
Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088]

Immunohistochemistry-Paraffin: Spectrin beta 2 Antibody [NBP1-86088] - Staining of human pancreas shows weak to moderate membranous positivity in exocrine glandular cells.
Spectrin beta 2 Antibody - BSA Free Western Blot: Spectrin beta 2 Antibody - BSA Free [NBP1-86088]

Western Blot: Spectrin beta 2 Antibody - BSA Free [NBP1-86088]

Analysis in human cell line WM-115.

Applications for Spectrin beta 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Spectrin beta 2

Spectrin Beta 2 is a major constituent of the erythrocyte skeleton. The membrane skeleton influences several cellular properties such as cell shape, restriction of mobility of the integral membrane protein exposed at the cell surface, and transmembrane movement of phospholipids and cholesterol.

Alternate Names

beta-fodrin, beta-G spectrin, Beta-II spectrin, beta-spectrin 2, beta-spectrin II, betaSpII, ELF, embryonic liver beta-fodrin, Fodrin beta chain, spectrin beta chain, brain 1, spectrin, beta, non-erythrocytic 1, Spectrin, non-erythroid beta chain 1, SPTB2

Gene Symbol

SPTBN1

Additional Spectrin beta 2 Products

Product Documents for Spectrin beta 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Spectrin beta 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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