Spectrin beta 3 Antibody (SPTBN2/1582)

Novus Biologicals | Catalog # NBP2-53236

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # SPTBN2/1582
View all beta-III Spectrin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa356-475) of human Spectrin beta 3 protein (exact sequence is proprietary) (Uniprot: O15020)

Localization

Cell Surface

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Theoretical MW

246 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-54488)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Spectrin beta 3 Antibody (SPTBN2/1582)

Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236]

Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236]

Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Western Blot Analysis of human HeLa cell lysate using Spectrin beta 3 Antibody (SPTBN2/1582).
Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236]

Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236]

Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Formalin-fixed, paraffin-embedded human Pancreas stained with Spectrin beta 3 Antibody (SPTBN2/1582).
Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236]

Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236]

Flow Cytometry: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Flow Cytometric Analysis of HeLa cells using Spectrin beta 3 Antibody (SPTBN2/1582). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236]

Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236]

Western Blot: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Analysis of HeLa and 293 Cell Lysates using Spectrin beta III Monoclonal Antibody (SPTBN2/1582).
Protein Array: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236]

Protein Array: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236]

Protein Array: Spectrin beta 3 Antibody (SPTBN2/1582) [NBP2-53236] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Spectrin beta 3 Antibody (SPTBN2/1582). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

Applications for Spectrin beta 3 Antibody (SPTBN2/1582)

Application
Recommended Usage

ELISA

2-4 ug/ml

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use Ab at 2-4ug/ml for coating. Order Ab without BSA.
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

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Advanced Features

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: beta-III Spectrin

Spectrins are principle components of a cell's membrane-cytoskeleton and are composed of two alpha and two beta spectrin subunits. The protein encoded by this gene (SPTBN2), is called spectrin beta non-erythrocytic 2 or beta-III spectrin. It is related to, but distinct from, the beta-II spectrin gene which is also known as spectrin beta non-erythrocytic 1 (SPTBN1). SPTBN2 regulates the glutamate signaling pathway by stabilizing the glutamate transporter EAAT4 at the surface of the plasma membrane.

Mutations in this gene cause a form of spinocerebellar ataxia, SCA5, that is characterized by neurodegeneration, progressive locomotor incoordination, dysarthria, and uncoordinated eye movements. Mutations in spectrins are a previously unknown cause of ataxia and neurodegenerative disease that affect membrane proteins involved in glutamate signaling. Spectrin-beta IIIs have been recognized as ataxia disease genes and their mutations cause spinocerebellar ataxia type 5 (SCA5).

Long Name

Spectrin Beta, Non-Erythrocytic 2

Alternate Names

GTRAP41, SCA5, SCAR14, Spectrin beta 3, Spectrin beta III, SPTBN2

Gene Symbol

SPTBN2

UniProt

O15020 (Human)

Additional beta-III Spectrin Products

Product Documents for Spectrin beta 3 Antibody (SPTBN2/1582)

Certificate of Analysis

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Product Specific Notices for Spectrin beta 3 Antibody (SPTBN2/1582)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Microtubules

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Protocols

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FAQs

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