Spectrin beta 3 Antibody - BSA Free
Novus Biologicals | Catalog # NB110-58346
![Western Blot: Spectrin beta 3 AntibodyBSA Free [NB110-58346]](https://resources.rndsystems.com/images/products/Spectrin-beta-3-Antibody-Western-Blot-NB110-58346-img0003.jpg "Western Blot: Spectrin beta 3 AntibodyBSA Free [NB110-58346]")
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
The epitope recognized by this antibody maps to a C-terminal region between residue 2340 and 2390 of Spectrin beta 3 using the numbering given in entry NP_008877.1
Reactivity Notes
Use in Mouse reported in scientific literature (PMID:32052742).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
271 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Spectrin beta 3 Antibody - BSA Free
Western Blot: Spectrin beta 3 AntibodyBSA Free [NB110-58346]
Western Blot: Spectrin beta 3 Antibody [NB110-58346] - Detection of Human Spectrin beta 3 on HeLa whole cell lysates using NB110-58346. Spectrin beta 3 was also immunoprecipitated by rabbit anti-Spectrin beta 3 antibody NB110-58783.![Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody - BSA Free [NB110-58346]](https://resources.rndsystems.com/images/products/Spectrin-beta-3-Antibody-Immunohistochemistry-Paraffin-NB110-58346-img0002.jpg "Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody - BSA Free [NB110-58346]")
Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody - BSA Free [NB110-58346]
Immunohistochemistry-Paraffin: Spectrin beta 3 Antibody [NB110-58346] - Detection of Spectrin beta 3 in human prostate epithelium.Applications for Spectrin beta 3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 32052742)
Immunohistochemistry
10-20 ug/ml
Immunohistochemistry-Paraffin
10-20 ug/ml
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: beta-III Spectrin
Long Name
Spectrin Beta, Non-Erythrocytic 2
Alternate Names
GTRAP41, SCA5, SCAR14, Spectrin beta 3, Spectrin beta III, SPTBN2
Entrez Gene IDs
6712 (Human)
Gene Symbol
SPTBN2
UniProt
Additional beta-III Spectrin Products
Product Documents for Spectrin beta 3 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Spectrin beta 3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Spectrin beta 3 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Spectrin beta 3 Antibody - BSA Free
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Q: Do you have stability tests done on NB110-58346 if left at RT for several hours ? Customer is doing automatic staining protocol where the antibody needs to stay several hours at RT and they do not get the nice results as with a manual staining, so we are wondering if the antibody would inactivate or get aggregated?
A: Unfortunately, we do not have any stability data on Spectrin beta 3 antibody with catalogue number NB110-58346, however, typical staining protocols involve a couple of hours incubation at room temperature or are performed overnight at 4C. Therefore, I am assuming that the customer should be OK by incubating tissues sections with NB110-58346 for little over a couple of hours without any problems. It may be noted however that the extended incubation with primary antibodies may lead to non-specific binding or overstaining.
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