SPG20 Antibody

Name: SPG20 Antibody
Brand: Novus Biologicals
SKU: NBP1-47203
Price: 419 USD
Availability: InStock

Novus Biologicals | Catalog # NBP1-47203

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all SPG20 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EASGTDVKQLDQGNK corresponding to internal region according to NP_055902.1.

Epitope

C-EASGTDVKQLDQGNK

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for SPG20 Antibody

Western Blot: SPG20 Antibody [NBP1-47203]

Western Blot: SPG20 Antibody [NBP1-47203] - (2ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: SPG20 Antibody [NBP1-47203]

Immunocytochemistry/Immunofluorescence: SPG20 Antibody [NBP1-47203] - (2ug/ml) staining of methanol-fixed HeLa before (left) and after (right) introduction of siRNA.

Applications for SPG20 Antibody

Application

Recommended Usage

Peptide ELISA

Detection limit 1:8000

Western Blot

1 - 3 ug/ml

Application Notes

IF: HeLa show dimished signals after knock down of expresssion by introduction of siRNA. WB: Approx. 70 kDa band observed in HeLa lysates (calculated MW of 72.8 kDa band according to NP_055902.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SPG20

The SPG20 gene encodes a protein containing a MIT (Microtubule Interacting and Trafficking molecule) domain, and is implicated in regulating endosomal trafficking and mitochondria function. The protein localizes to mitochondria and partially co-localizes with

Alternate Names

KIAA0610SPARTIN, spastic paraplegia 20 (Troyer syndrome), Spastic paraplegia 20 protein, TAHCCP1spartin, Trans-activated by hepatitis C virus core protein 1

Entrez Gene IDs

23111 (Human)

Gene Symbol

SPART

UniProt

NP_055902.1 (Human)

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Product Documents for SPG20 Antibody

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Certificate of Analysis

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Product Specific Notices for SPG20 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SPG20 Antibody

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