Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6)

Novus Biologicals | Catalog # NBP2-45944

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Monkey

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1A6
View all Sphingosine Kinase 1/SPHK1 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human SPHK1(NP_068807) produced in HEK293T cell.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:33077711).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

43.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6)

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1A6) [NBP2-45944] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SPHK1.
Immunocytochemistry/ Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Immunocytochemistry/ Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944] - Analysis of HeLa cells.
Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (1A6) [NBP2-45944] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)
Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Western Blot: Sphingosine Kinase 1/SPHK1 Antibody (1A6) [NBP2-45944] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Immunocytochemistry/ Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Immunocytochemistry/Immunofluorescence: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY Sphingosine Kinase 1/SPHK1.
Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6) [NBP2-45944]

Immunohistochemistry: Sphingosine Kinase 1/SPHK1 Antibody (1A6) [NBP2-45944] - Analysis of Adenocarcinoma of Human breast tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:400-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Sphingosine Kinase 1/SPHK1

Sphingosine-1-phosphate (SPP) is a novel lipid messenger with both intracellular and extracellular functions. Intracellularly, it regulates proliferation and survival, and extracellularly, it is a ligand for EDG1 (MIM 601974). Various stimuli increase cellular levels of SPP by activation of sphingosine kinase (SPHK), the enzyme that catalyzes the phosphorylation of sphingosine. Competitive inhibitors of SPHK block formation of SPP and selectively inhibit cellular proliferation induced by a variety of factors, including platelet-derived growth factor (e.g., MIM 173430) and serum.[supplied by OMIM]

Alternate Names

SK1, SPK1

Gene Symbol

SPHK1

Additional Sphingosine Kinase 1/SPHK1 Products

Product Documents for Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6)

Certificate of Analysis

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Product Specific Notices for Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Sphingosine Kinase 1/SPHK1 Antibody (OTI1A6)

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Protocols

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FAQs

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Associated Pathways

VEGF - VEGF R2 Signaling Pathways VEGF - VEGF R2 Signaling Pathway Thumbnail