Sphingosine Kinase 2/SPHK2 Antibody
Novus Biologicals | Catalog # NBP3-23423
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Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
Sphingosine Kinase 2/SPHK2 (N-terminal region) synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human Sphingosine Kinase 2/SPHK2. This peptide sequence is highly conserved in rat and mouse Sphingosine Kinase 2/SPHK2 proteins, and has no homology to SK1. [UniProt# Q9NRA0]
Specificity
The antibody detects 70 kDa proteins corresponding to the molecular mass of Sphingosine Kinase 2/SPHK2 on SDS-PAGE immunoblots of human recombinant Sphingosine Kinase 2/SPHK2 and endogenous Sphingosine Kinase 2/SPHK2 in human HeLa cells.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for Sphingosine Kinase 2/SPHK2 Antibody
Western Blot: Sphingosine Kinase 2/SPHK2 Antibody [NBP3-23423] -
Western Blot: Sphingosine Kinase 2/SPHK2 Antibody [NBP3-23423] - Western blot of human recombinant Sphingosine Kinase 2/SPHK2 (lanes 1-4) and HeLa treated with calyculin A (lanes 5-8). The blots were untreated (lane 1, 3, 5 & 7) or treated with lambda phosphatase (lane 2, 4, 6 & 8), then probed with anti-Sphingosine Kinase 2/SPHK2 (N-terminal region) (lanes 1, 2, 5 & 6) or anti-Sphingosine Kinase 2/SPHK2 (Thr-578) (lanes 3, 4, 7, & 8).Applications for Sphingosine Kinase 2/SPHK2 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunoprecipitation
1:100
Western Blot
1:250
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Sphingosine Kinase 2/SPHK2
Alternate Names
SK2, SPK2
Gene Symbol
SPHK2
Additional Sphingosine Kinase 2/SPHK2 Products
Product Documents for Sphingosine Kinase 2/SPHK2 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Sphingosine Kinase 2/SPHK2 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
