SPR Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87060

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SPR Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LEEPNVRVLNYAPGPLDTDMQQLARETSVDPDMRKGLQELKAKGKLVDCKVSAQKLLSLLEKDEFKSGAHVDFY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPR Antibody - BSA Free

Western Blot: SPR Antibody [NBP1-87060]

Western Blot: SPR Antibody [NBP1-87060]

Western Blot: SPR Antibody [NBP1-87060] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue
Immunocytochemistry/ Immunofluorescence: SPR Antibody [NBP1-87060]

Immunocytochemistry/ Immunofluorescence: SPR Antibody [NBP1-87060]

Immunocytochemistry/Immunofluorescence: SPR Antibody [NBP1-87060] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
SPR Antibody - BSA Free Immunohistochemistry-Paraffin: SPR Antibody - BSA Free [NBP1-87060]

Immunohistochemistry-Paraffin: SPR Antibody - BSA Free [NBP1-87060]

Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Applications for SPR Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPR

SPR encodes an aldo-keto reductase that catalyzes the NADPH-dependent reduction of pteridine derivatives and is important in the biosynthesis of tetrahydrobiopterin (BH4). Mutations in this gene result in DOPA-responsive dystonia due to sepiaterin reductase deficiency. A pseudogene has been identified on chromosome 1.

Long Name

Sepiapterin Reductase

Alternate Names

EC 1.1.1.153, Gm10328, SDR38C1

Gene Symbol

SPR

Additional SPR Products

Product Documents for SPR Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPR Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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