SPRY1 Antibody (3H4) - Azide and BSA Free

Novus Biologicals | Catalog # H00010252-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 3H4

Format

Azide and BSA Free
View all SPRY1 Primary Antibodies »

Product Specifications

Immunogen

SPRY1 (NP_005832, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MDPQNQHGSGSSLVVIQQPSLDSRQRLDYEREIQPTAILSLDQIKAIRGSNEYTEGPSVVKRPAPRTAPRQEKHERTHEIIPINVNNNYEHRHTSHLGHAVLPSNARGPI

Specificity

SPRY1 - sprouty homolog 1, antagonist of FGF signaling (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SPRY1 Antibody (3H4) - Azide and BSA Free

Western Blot: SPRY1 Antibody (3H4) [H00010252-M01]

Western Blot: SPRY1 Antibody (3H4) [H00010252-M01]

Western Blot: SPRY1 Antibody (3H4) [H00010252-M01] - SPRY1 monoclonal antibody (M01), clone 3H4 Analysis of SPRY1 expression in HepG2.
Immunohistochemistry-Paraffin: SPRY1 Antibody (3H4) [H00010252-M01]

Immunohistochemistry-Paraffin: SPRY1 Antibody (3H4) [H00010252-M01]

Immunohistochemistry-Paraffin: SPRY1 Antibody (3H4) [H00010252-M01] - Analysis of monoclonal antibody to SPRY1 on formalin-fixed paraffin-embedded human heart. Antibody concentration 3 ug/ml.
Western Blot: SPRY1 Antibody (3H4) [H00010252-M01]

Western Blot: SPRY1 Antibody (3H4) [H00010252-M01]

Western Blot: SPRY1 Antibody (3H4) [H00010252-M01] - Analysis of SPRY1 expression in transfected 293T cell line by SPRY1 monoclonal antibody (M01), clone 3H4.Lane 1: SPRY1 transfected lysate(35.1 KDa).Lane 2: Non-transfected lysate.
ELISA: SPRY1 Antibody (3H4) [H00010252-M01]

ELISA: SPRY1 Antibody (3H4) [H00010252-M01]

ELISA: SPRY1 Antibody (3H4) [H00010252-M01] - Detection limit for recombinant GST tagged SPRY1 is approximately 0.03ng/ml as a capture antibody.

Applications for SPRY1 Antibody (3H4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SPRY1

Sprouty may function as an antagonist of fibroblast growth factor (FGF) pathways and may negatively modulate respiratory organogenesis

Long Name

Sprouty Homolog 1

Alternate Names

Drosophila, homolog of, 1 (antagonist of FGF signaling), sprouty homolog 1, antagonist of FGF signaling (Drosophila), spry-1

Entrez Gene IDs

10252 (Human)

Gene Symbol

SPRY1

OMIM

602465 (Human)

UniProt

NP_005832 (Human)

Additional SPRY1 Products

Product Documents for SPRY1 Antibody (3H4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SPRY1 Antibody (3H4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

FGF Family

Citations for SPRY1 Antibody (3H4) - Azide and BSA Free

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Protocols

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FAQs

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