SPTLC1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81737

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SPTLC1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KTYKLQERSDLTVKEKEELIEEWQPEPLVPPVPKDHPALNYNIVSGPPSHKTVVNGKECINFASFNFLGLLDNPRVKAAALASLKKYGVGTCGPR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SPTLC1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SPTLC1 Antibody [NBP1-81737]

Immunohistochemistry-Paraffin: SPTLC1 Antibody [NBP1-81737]

Immunohistochemistry-Paraffin: SPTLC1 Antibody [NBP1-81737] - Staining of human breast shows moderate cytoplasmic positivity in glandular cells.
SPTLC1 Antibody Western Blot: SPTLC1 Antibody Antibody [NBP1-81737]

Western Blot: SPTLC1 Antibody Antibody [NBP1-81737]

Analysis using NBP1-81737 (A) shows similar pattern to independent antibody NBP2-56477 (B).

Applications for SPTLC1 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SPTLC1

Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified. (provided by RefSeq)

Long Name

Serine palmitoyltransferase 1

Alternate Names

LCB 1, LCB1, SPT 1, SPT1

Gene Symbol

SPTLC1

Additional SPTLC1 Products

Product Documents for SPTLC1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SPTLC1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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