SRC1 Antibody

Novus Biologicals | Catalog # NB300-541

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Amphibian, Avian

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SRC1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues S(8) S D P A N P D S H K R K G S P C(24) of human SRC-1.

Reactivity Notes

Chicken reactivity reported in scientific literature (see Charlier et all 2005). Avian reactivity reported in scientific literature (see Charlier et all 2006), Amphibian reactivity reported in scientific literature (see Kerver et all 2013).

Localization

Nuclear

Specificity

Detects steroid receptor coactivator-1 (SRC-1).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SRC1 Antibody

Immunocytochemistry/ Immunofluorescence: SRC1 Antibody [NB300-541]

Immunocytochemistry/ Immunofluorescence: SRC1 Antibody [NB300-541]

Immunocytochemistry/Immunofluorescence: SRC1 Antibody [NB300-541] - Immunolocalization of HeLa cells.
Immunohistochemistry-Frozen: SRC1 Antibody [NB300-541]

Immunohistochemistry-Frozen: SRC1 Antibody [NB300-541]

Immunohistochemistry-Frozen: SRC1 Antibody [NB300-541] - Staining of SRC-1 in the Dentate Gyrus region of the rat brain.

Applications for SRC1 Antibody

Application
Recommended Usage

Flow Cytometry

3 - 5 ug / 10^6 cells

Immunocytochemistry/ Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry

1:10 - 1:500

Immunohistochemistry-Frozen

1 ug/ml

Western Blot

1 ug/ml
Application Notes
WB: Detects an approx. 165 kDa protein representing SRC 1 and a smaller, unknown protein at ~40 kDa from HeLa cell lysate. ICC: Staining of SRC 1 in HeLa cells results in nuclear staining. May be useful in IF (Privalsky et al 2009). Use in immunoprecipitation reported in scientific literature (PMID: 19487700).

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SRC1

Steroid and thyroid hormones and retinoic acid regulate a complex array of gene expression activity via intracellular receptor transcription factors belonging to the ligand dependent nuclear receptor superfamily. Adding to the complexity of function of these transcription factors are associated proteins known as coactivators and corepressors which, as their names suggest, enhance or depress transcriptional activity of the nuclear receptor with which they associate. One such coactivator is Steroid Receptor Coactivator-1 (SRC 1).SRC 1 has been shown to interact with and stimulate the transcriptional activity of estrogen, progesterone, retinoic acid, thyroid and glucocorticoid receptors, as well as retinoic X receptor (RXR) in a ligand dependent manner. The amino terminal region of SRC 1 contains a PAS-A-basic helix-loop-helix homology domain which has previously been shown to be dimerization domains in other nuclear transcription factors including the aryl hydrocarbon (Ah) receptor and its heterodimerization partner, ARNT. The carboxy-terminal region of SRC 1 has been shown to possess histone acetyltransferase activity specific primarily for histones H3 & H4.

Long Name

Nuclear receptor coactivator 1

Alternate Names

bHLHe74, NCoA-1, NCOA1, Protein Hin-2, RIP160

Entrez Gene IDs

8648 (Human); 20779 (Mouse)

Gene Symbol

NCOA1

UniProt

Q15788 (Human)

Additional SRC1 Products

Product Documents for SRC1 Antibody

Certificate of Analysis

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Product Specific Notices for SRC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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