SREBP2 Antibody

Novus Biologicals | Catalog # NB100-74543

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SREBP2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues A(843) I S W L Q G D D A A V R S H(857) of rat SREBP 2.

Reactivity Notes

Mouse reactivity reported in scientific literature (see J Biol Chem. 2007 Mar).

Localization

Precursor form found in ER, shuttles to Golgi for activation by proteolytic cleavage, mature form in nucleus.

Specificity

SREBP2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SREBP2 Antibody

Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody [NB100-74543]

Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody [NB100-74543]

Immunocytochemistry/Immunofluorescence: SREBP2 Antibody [NB100-74543] - Analysis of SREBP2 (green) showing staining in the cytoplasm of HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SREBP2 polyclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody [NB100-74543]

Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody [NB100-74543]

Immunocytochemistry/Immunofluorescence: SREBP2 Antibody [NB100-74543] - Analysis of SREBP2 (green) showing staining in the cytoplasm of PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SREBP2 polyclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody [NB100-74543]

Immunocytochemistry/ Immunofluorescence: SREBP2 Antibody [NB100-74543]

Immunocytochemistry/Immunofluorescence: SREBP2 Antibody [NB100-74543] - Analysis of SREBP2 (green) showing staining in the cytoplasm of L6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a SREBP2 polyclonal antibody in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI.
SREBP2 Antibody

Western Blot: SREBP2 Antibody [NB100-74543] -

The expression of molecules regulating cholesterol synthesis in the brains of WT and HIV-Tg rats. (A) WBs images showing the expression of SREBP1, SREBP2, PLIN2, HMGCR in the HP of WT and HIV-Tg rats; (B) statistical analysis of SREBP1 levels in the HP of WT and HIV-Tg rats; (C) statistical analysis of SREBP2 levels in the HP of WT and HIV-Tg rats (* P < 0.05); (D) statistical analysis of PLIN2 levels in the HP of WT and HIV-Tg rats; (E) statistical analysis of HMGCR levels in the HP of WT and HIV-Tg rats; (F) WBs images showing the expression of SREBP1, SREBP2, PLIN2, HMGCR in the PFc of WT and HIV-Tg rats; (G) statistical analysis of SREBP1 levels in the PFc of WT and HIV-Tg rats; (H) statistical analysis of SREBP2 levels in the PFc of WT and HIV-Tg rats; (I) statistical analysis of PLIN2 levels in the HP of WT and HIV-Tg rats (* P < 0.05); (J) statistical analysis of HMGCR levels in the HP of WT and HIV-Tg rats (* P < 0.05). Each group contains 5 rats, unpaired two tail t-test was used for statistical analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38377024), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
SREBP2 Antibody

Western Blot: SREBP2 Antibody [NB100-74543] -

The expression of molecules regulating cholesterol synthesis in the brains of WT and HIV-Tg rats. (A) WBs images showing the expression of SREBP1, SREBP2, PLIN2, HMGCR in the HP of WT and HIV-Tg rats; (B) statistical analysis of SREBP1 levels in the HP of WT and HIV-Tg rats; (C) statistical analysis of SREBP2 levels in the HP of WT and HIV-Tg rats (* P < 0.05); (D) statistical analysis of PLIN2 levels in the HP of WT and HIV-Tg rats; (E) statistical analysis of HMGCR levels in the HP of WT and HIV-Tg rats; (F) WBs images showing the expression of SREBP1, SREBP2, PLIN2, HMGCR in the PFc of WT and HIV-Tg rats; (G) statistical analysis of SREBP1 levels in the PFc of WT and HIV-Tg rats; (H) statistical analysis of SREBP2 levels in the PFc of WT and HIV-Tg rats; (I) statistical analysis of PLIN2 levels in the HP of WT and HIV-Tg rats (* P < 0.05); (J) statistical analysis of HMGCR levels in the HP of WT and HIV-Tg rats (* P < 0.05). Each group contains 5 rats, unpaired two tail t-test was used for statistical analysis. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38377024), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for SREBP2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20 - 1:200

Western Blot

1:100 - 1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SREBP2

Sterol regulatory element-binding proteins (SREBPs) are transcription factors that are members of the basic helix-loop-helix leucine zipper family of DNA binding proteins. Three isoforms have been identified in mammalian tissues that vary in structure, regulation, and function. SREBP-1a and SREBP-1c (originally cloned as ADD1) are protein products of alternative promoter usage of the SREBP-1 gene. The third isoform is transcribed from a different gene, SREBP-2. SREBPs are present as 120 kDa inactive precursors in the endoplasmic reticulum (ER) membrane. Upon activation, the SREBP protein is translocated to the Golgi and proteolytic cleavage occurs resulting in a mature transcriptionally active 60-78 kDa fragment. In liver and adipose tissues, SREBPs have a significant influence on lipid and cholesterol accumulation by inducing the transcription of genes involved in these processes. While SREBP-1 is thought to be more important in regulating the expression of genes involved in triglyceride synthesis and accumulation, SREBP-2 has been more closely linked to those involved in cholesterol synthesis and accumulation.

Long Name

Sterol Regulatory Element Binding Transcription Factor 2

Alternate Names

bHLhd2, SREBF2

Entrez Gene IDs

20788 (Mouse); 300095 (Rat)

Gene Symbol

SREBF2

UniProt

Q3T1I5 (Rat)

Additional SREBP2 Products

Product Documents for SREBP2 Antibody

Certificate of Analysis

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Product Specific Notices for SREBP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SREBP2 Antibody

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Protocols

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Associated Pathways

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