SRGAP1 Antibody (5D10) - Azide and BSA Free

Novus Biologicals | Catalog # H00057522-M07

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5D10

Format

Azide and BSA Free
View all SRGAP1 Primary Antibodies »

Product Specifications

Immunogen

SRGAP1 (NP_065813, 952 a.a. ~ 1050 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLDPETIAQDIEETMNTALNELRELERQSTAKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQL

Reactivity Notes

This product is reactive against Human,Mouse.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SRGAP1 Antibody (5D10) - Azide and BSA Free

Western Blot: SRGAP1 Antibody (5D10) [H00057522-M07]

Western Blot: SRGAP1 Antibody (5D10) [H00057522-M07]

Western Blot: SRGAP1 Antibody (5D10) [H00057522-M07] - Analysis of SRGAP1 expression in IMR-32 (Cat # L008V1).
Immunocytochemistry/ Immunofluorescence: SRGAP1 Antibody (5D10) [H00057522-M07]

Immunocytochemistry/ Immunofluorescence: SRGAP1 Antibody (5D10) [H00057522-M07]

Immunocytochemistry/Immunofluorescence: SRGAP1 Antibody (5D10) [H00057522-M07] - Analysis of monoclonal antibody to SRGAP1 on HeLa cell. Antibody concentration 10 ug/ml
Western Blot: SRGAP1 Antibody (5D10) [H00057522-M07]

Western Blot: SRGAP1 Antibody (5D10) [H00057522-M07]

Western Blot: SRGAP1 Antibody (5D10) [H00057522-M07] - Analysis of SRGAP1 expression in NIH/3T3 (Cat # L018V1).
Sandwich ELISA: SRGAP1 Antibody (5D10) [H00057522-M07] - Detection limit for recombinant GST tagged SRGAP1 is 0.03 ng/ml as a capture antibody.

Applications for SRGAP1 Antibody (5D10) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is useful for ELISA, Western Blot

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SRGAP1

SRGAP1/ARHGAP13 is a member of the srGAP family of proteins that contain a RhoGAP domain, an SH3 domain, and a Fes/CIP4 homology domain and interact with the intracellular domain of roundabout 1 (Robo1). Robo1 is the receptor for Slit signaling and is involved in neuronal cell migration and axon tract positioning. SRGAP1/ARHGAP13 functions to bind and decrease the level of active Cdc42 and RhoA small GTPases during Slit-Robo signaling. Alternate names for SRGAP1/ARHGAP13 include Rho GTPase-activating protein 13, and KIAA1304.

Alternate Names

ARHGAP13srGAP1, FLJ22166, KIAA1304SLIT-ROBO Rho GTPase-activating protein 1, Rho GTPase-activating protein 13, SLIT-ROBO Rho GTPase activating protein 1

Gene Symbol

SRGAP1

UniProt

NP_065813 (Human)

Additional SRGAP1 Products

Product Documents for SRGAP1 Antibody (5D10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SRGAP1 Antibody (5D10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SRGAP1 Antibody (5D10) - Azide and BSA Free

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Protocols

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FAQs

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