SRGAP1 Antibody (5D2) - Azide and BSA Free

Novus Biologicals | Catalog # H00057522-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5D2

Format

Azide and BSA Free
View all SRGAP1 Primary Antibodies »

Product Specifications

Immunogen

SRGAP1 (NP_065813, 952 a.a. ~ 1050 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLDPETIAQDIEETMNTALNELRELERQSTAKHAPDVVLDTLEQVKNSPTPATSTESLSPLHNVALRSSEPQIRRSTSSSSDTMSTFKPMVAPRMGVQL

Specificity

SRGAP1 - SLIT-ROBO Rho GTPase activating protein 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SRGAP1 Antibody (5D2) - Azide and BSA Free

Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03]

Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03]

Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03] - SRGAP1 expression in NIH/3T3 ( Cat # L018V1 ).
Immunohistochemistry-Paraffin: SRGAP1 Antibody (5D2) [H00057522-M03]

Immunohistochemistry-Paraffin: SRGAP1 Antibody (5D2) [H00057522-M03]

Immunohistochemistry-Paraffin: SRGAP1 Antibody (5D2) [H00057522-M03] - Analysis of monoclonal antibody to SRGAP1 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml
Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03]

Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03]

Western Blot: SRGAP1 Antibody (5D2) [H00057522-M03] - SRGAP1 expression in IMR-32 ( Cat # L008V1 ).
ELISA: SRGAP1 Antibody (5D2) [H00057522-M03]

ELISA: SRGAP1 Antibody (5D2) [H00057522-M03]

ELISA: SRGAP1 Antibody (5D2) [H00057522-M03] - Detection limit for recombinant GST tagged SRGAP1 is approximately 0.1ng/ml as a capture antibody.

Applications for SRGAP1 Antibody (5D2) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SRGAP1

GTPase-activating protein for RhoA and Cdc42 small GTPases. Together with CDC42 seems to be involved in thepathway mediating the repulsive signaling of Robo and Slit proteins in neuronal migration. SLIT2, probably throughinteraction with ROBO1, increases the interaction of SRGAP1 with ROBO1 and inactivates CDC42

Alternate Names

ARHGAP13srGAP1, FLJ22166, KIAA1304SLIT-ROBO Rho GTPase-activating protein 1, Rho GTPase-activating protein 13, SLIT-ROBO Rho GTPase activating protein 1

Entrez Gene IDs

57522 (Human)

Gene Symbol

SRGAP1

OMIM

606523 (Human)

UniProt

NP_065813 (Human)

Additional SRGAP1 Products

Product Documents for SRGAP1 Antibody (5D2) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SRGAP1 Antibody (5D2) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SRGAP1 Antibody (5D2) - Azide and BSA Free

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Protocols

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FAQs

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