SSH3 Antibody (6F9) - Azide and BSA Free

Novus Biologicals | Catalog # H00054961-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 6F9

Format

Azide and BSA Free
View all SSH3 Primary Antibodies »

Product Specifications

Immunogen

SSH3 (NP_060327, 293 a.a. ~ 391 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SKEIRQALELRLGLPLQQYRDFIDNQMLLLVAQRDRASRIFPHLYLGSEWNAANLEELQRNRVTHILNMAREIDNFYPERFTYHNVRLWDEESAQLLPH

Specificity

SSH3 - slingshot homolog 3 (Drosophila)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SSH3 Antibody (6F9) - Azide and BSA Free

Western Blot: SSH3 Antibody (6F9) [H00054961-M01]

Western Blot: SSH3 Antibody (6F9) [H00054961-M01]

Western Blot: SSH3 Antibody (6F9) [H00054961-M01] - SSH3 monoclonal antibody (M01), clone 6F9 Analysis of SSH3 expression in A-431.
Immunocytochemistry/ Immunofluorescence: SSH3 Antibody (6F9) [H00054961-M01]

Immunocytochemistry/ Immunofluorescence: SSH3 Antibody (6F9) [H00054961-M01]

Immunocytochemistry/Immunofluorescence: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of monoclonal antibody to SSH3 on A-431 cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: SSH3 Antibody (6F9) [H00054961-M01]

Immunohistochemistry-Paraffin: SSH3 Antibody (6F9) [H00054961-M01]

Immunohistochemistry-Paraffin: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of monoclonal antibody to SSH3 on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 1 ~ 10 ug/ml.
Western Blot: SSH3 Antibody (6F9) [H00054961-M01]

Western Blot: SSH3 Antibody (6F9) [H00054961-M01]

Western Blot: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of SSH3 expression in transfected 293T cell line by SSH3 monoclonal antibody (M01), clone 6F9.Lane 1: SSH3 transfected lysate(73 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: SSH3 Antibody (6F9) [H00054961-M01]

Immunoprecipitation: SSH3 Antibody (6F9) [H00054961-M01]

Immunoprecipitation: SSH3 Antibody (6F9) [H00054961-M01] - Analysis of SSH3 transfected lysate using anti-SSH3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SSH3 monoclonal antibody.
Sandwich ELISA: SSH3 Antibody (6F9) [H00054961-M01] - Detection limit for recombinant GST tagged SSH3 is approximately 0.3ng/ml as a capture antibody.

Applications for SSH3 Antibody (6F9) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SSH3

The ADF (actin-depolymerizing factor)/cofilin family (see MIM 601442) is composed of stimulus-responsive mediators of actin dynamics. ADF/cofilin proteins are inactivated by kinases such as LIM domain kinase-1 (LIMK1; MIM 601329). The SSH family appears to play a role in actin dynamics by reactivating ADF/cofilin proteins in vivo (Niwa et al., 2002 [PubMed 11832213]).[supplied by OMIM]

Alternate Names

EC 3.1.3.16, EC 3.1.3.48, FLJ10928, FLJ20515, FLJ42240, hSSH-3L, protein phosphatase Slingshot homolog 3, slingshot 3, slingshot homolog 3 (Drosophila), SSH3, SSH3L, SSH-3L, SSH-like protein 3

Entrez Gene IDs

54961 (Human)

Gene Symbol

SSH3

OMIM

606780 (Human)

UniProt

NP_060327 (Human)

Additional SSH3 Products

Product Documents for SSH3 Antibody (6F9) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SSH3 Antibody (6F9) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SSH3 Antibody (6F9) - Azide and BSA Free

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Protocols

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FAQs

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