SSX2 Antibody (OTI4D10) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74368

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4D10

Format

Azide and BSA Free
View all SSX2 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SSX2 expression vector

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

21.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SSX2 Antibody (OTI4D10) - Azide and BSA Free

Western Blot: SSX2 Antibody (OTI4D10)Azide and BSA Free [NBP2-74368]

Western Blot: SSX2 Antibody (OTI4D10)Azide and BSA Free [NBP2-74368]

Western Blot: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSX2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSX2.
Immunocytochemistry/ Immunofluorescence: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunocytochemistry/ Immunofluorescence: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunocytochemistry/Immunofluorescence: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSX2.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded pancreas tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded Carcinoma of bladder tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded Carcinoma of thyroid tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded colon tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded endometrium tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - Staining of paraffin-embedded Ovary tissue using anti-SSX2 mouse monoclonal antibody.
Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368]

Immunohistochemistry: SSX2 Antibody (OTI4D10) - Azide and BSA Free [NBP2-74368] - SSX2 Antibody (4D10) [NBP1-48008

Applications for SSX2 Antibody (OTI4D10) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SSX2

The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t(X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are probably responsible for transforming activity. Two transcript variants encoding distinct isoforms have been identified for this gene.

Alternate Names

Cancer/testis antigen 5.2, cancer/testis antigen family 5, member 2a, CT5.2a, HD21, HOM-MEL-40, MGC119055, MGC15364, MGC3884, protein SSX2, sarcoma, synovial, X-chromosome-related 2, SSX, SSX2A, SSX2B, synovial sarcoma, X breakpoint 2B, synovial sarcoma, X breakpoint 2CT5.2, Tumor antigen HOM-MEL-40

Gene Symbol

SSX2

Additional SSX2 Products

Product Documents for SSX2 Antibody (OTI4D10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SSX2 Antibody (OTI4D10) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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