STAG2 Antibody (JE57-34)

Western Blot: STAG2 Antibody (JE57-34) [NBP3-32925]

Western Blot: STAG2 Antibody (JE57-34) [NBP3-32925] - Western blot analysis of STAG2 on different lysates with Rabbit anti-STAG2 antibody (NBP3-32925) at 1/2,000 dilution.

Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: PANC-1 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF7 cell lysate
Lane 7: K-562 cell lysate
Lane 8: HCT 116 cell lysate
Lane 9: COS-1 cell lysate
Lane 10: NIH/3T3 cell lysate
Lane 11: RAW264.7 cell lysate
Lane 12: C6 cell lysate
Lane 13: mouse spleen tissue lysate
Lane 14: rat spleen tissue lysate

Cell lysates/proteins at 20 ug/Lane.
Tissues lysates/proteins at 40 ug/Lane.

Predicted band size:141 kDa
Observed band size: 141 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32925) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: STAG2 Antibody (JE57-34) [NBP3-32925]

Immunohistochemistry: STAG2 Antibody (JE57-34) [NBP3-32925] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-STAG2 antibody (NBP3-32925) at 1/2,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32925) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: STAG2 Antibody (JE57-34) [NBP3-32925]

Immunocytochemistry/ Immunofluorescence: STAG2 Antibody (JE57-34) [NBP3-32925] - Immunocytochemistry analysis of RAW264.7 cells labeling STAG2 with Rabbit anti-STAG2 antibody (NBP3-32925) at 1/250 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-STAG2 antibody (NBP3-32925) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.