![Western Blot: STAM-1 Antibody [NBP3-20227]](https://resources.rndsystems.com/images/products/NBP3-20227_Western-Blot-STAM-1-Antibody-NBP3-20227-1ug-ml-staining-of-K562-A-and-0-5ug-ml-MCF7-B-cell-lysate-35ug-protein-in-RIPA-buffer-Detected-by-chemiluminescence--5420231130454.jpg "Western Blot: STAM-1 Antibody [NBP3-20227]")
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Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-KTEKKTVQFSDD, from the internal region of the protein sequence according to NP_003464.1; NP_001311216.1; NP_001311212.1; NP_001311215.1; NP_001311211.1; NP_001311217.1.
Specificity
This antibody is expected to recognize usoforms a-f. No cross-reactivity expected with STAM2.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for STAM-1 Antibody
Western Blot: STAM-1 Antibody [NBP3-20227]
Western Blot: STAM-1 Antibody [NBP3-20227] - (1ug/ml) staining of K562 (A) and (0.5ug/ml) MCF7 (B) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.![Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227]](https://resources.rndsystems.com/images/products/NBP3-20227_Immunohistochemistry-Paraffin-STAM-1-Antibody-NBP3-20227-6ug-ml-staining-of-paraffin-embedded-Human-Testis-Heat-induced-antigen-retrieval-with-citrate-buffer-pH-6-HRP-staining--5420231130457.jpg "Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227]")
Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227]
Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227] - (6ug/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.![Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227]](https://resources.rndsystems.com/images/products/NBP3-20227_Immunohistochemistry-Paraffin-STAM-1-Antibody-NBP3-20227-Negative-Control-showing-staining-of-paraffin-embedded-Human-Testis-with-no-primary-antibody--5420231130456.jpg "Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227]")
Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227]
Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP3-20227] - Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.Applications for STAM-1 Antibody
Application
Recommended Usage
Immunohistochemistry-Paraffin
4-6 ug/ml
Peptide ELISA
Detection limit 1:16000
Western Blot
0.3-1 ug/ml
Application Notes
Western blot: Approx. 60kDa band observed in lysates of cell line K562, and approx. 55kDa in lysates of cell line MCF7, (calculated MW of 59.2kDa according to NP_003464.1 and 55.7kDa according to NP_001311211.1). Primary incubation 1 hour at room temperature.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: STAM-1
Long Name
Signal Transducing Adaptor Molecule 1
Alternate Names
HSE1 Homolog, STAM, STAM1
Gene Symbol
STAM
Additional STAM-1 Products
Product Documents for STAM-1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for STAM-1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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