Stargazin Antibody (HL2268) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25697

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2268 expressed in HEK293

Format

Azide and BSA Free
View all Stargazin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of mouse Stargazin.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Stargazin Antibody (HL2268) - Azide and BSA Free

Stargazin Antibody (HL2268) - Azide and BSA Free

Western Blot: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] -

Western Blot: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Stargazin Antibody (HL2268) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] -

Immunocytochemistry/Immunofluorescence: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Stargazin antibody [HL2268] detects Stargazin protein at cell membrane and synapse by immunofluorescent analysis. Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stargazin stained by Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:250. Red: Tau, a axon marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Stargazin Antibody (HL2268) - Azide and BSA Free

Immunohistochemistry-Paraffin: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] -

Immunohistochemistry-Paraffin: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Stargazin antibody [HL2268] detects Stargazin protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. Stargazin stained by Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Stargazin Antibody (HL2268) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] -

Immunocytochemistry/Immunofluorescence: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Stargazin antibody [HL2268] detects Stargazin protein at cytoplasm by immunofluorescent analysis. Sample: SH-SY5Y cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Stargazin stained by Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:500. Blue: Fluoroshield with DAPI.
Stargazin Antibody (HL2268) - Azide and BSA Free

Western Blot: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] -

Western Blot: Stargazin Antibody (HL2268) - Azide and BSA Free [NBP3-25697] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Stargazin Antibody (HL2268) - Azide and BSA Free

Western Blot: Stargazin Antibody (HL2268) [NBP3-25697] -

Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Stargazin antibody [HL2268] (NBP3-25697) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Applications for Stargazin Antibody (HL2268) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Stargazin

Stargazin is a member of the transmembrane AMPAR regulatory proteins (TARP) family and is involved in glutamate receptor trafficking. It has been recently demonstrated (Tomita et al., 2005; Priel et al., 2005) that the interaction between stargazin and AMPA receptors is critical for the correct localization of the receptors at the synapse. Phosphorylation of the stargazin protein at Thr 321 by Protein Kinase A regulates its interaction with PSD-95 and synaptic targeting of AMPA receptors (Choi et al., 2002). Stargazin mutant mice are utilized as a model for absence epilepsy.

Long Name

Voltage-dependent calcium channel gamma-2 subunit

Alternate Names

CACNG2, TARP gamma-2

Gene Symbol

CACNG2

Additional Stargazin Products

Product Documents for Stargazin Antibody (HL2268) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Stargazin Antibody (HL2268) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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