STAT4 Antibody (10H10O9)

Novus Biologicals | Catalog # NBP3-16425

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10H10O9 expressed in HEK293
View all STAT4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 649-748 of human STAT4 (NP_003142.1). MAENIPENPLKYLYPDIPKDKAFGKHYSSQPCEVSRPTERGDKGYVPSVFIPISTIRSDSTEPHSPSDLLPMSPSVYAVLRENLSPTTIETAMKSPYSAE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for STAT4 Antibody (10H10O9)

Western Blot: STAT4 Antibody (10H10O9) [NBP3-16425]

Western Blot: STAT4 Antibody (10H10O9) [NBP3-16425]

Western Blot: STAT4 Antibody (10H10O9) [NBP3-16425] - Western blot analysis of extracts of various cell lines, using STAT4 Rabbit mAb (NBP3-16425) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: STAT4 Antibody (10H10O9) [NBP3-16425]

Immunocytochemistry/ Immunofluorescence: STAT4 Antibody (10H10O9) [NBP3-16425]

Immunocytochemistry/Immunofluorescence: STAT4 Antibody (10H10O9) [NBP3-16425] - Immunofluorescence analysis of C6 cells using STAT4 Rabbit mAb (NBP3-16425) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
STAT4 Antibody (10H10O9)

Immunocytochemistry/ Immunofluorescence: STAT4 Antibody (10H10O9) [NBP3-16425] -

Immunocytochemistry/ Immunofluorescence: STAT4 Antibody (10H10O9) [NBP3-16425] - Immunofluorescence analysis of NIH-3T3 cells using STAT4 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for STAT4 Antibody (10H10O9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STAT4

STAT4 is encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.

Long Name

Signal Transducer and Activator of Transcription 4

Alternate Names

signal transducer and activator of transcription 4, SLEB11

Gene Symbol

STAT4

Additional STAT4 Products

Product Documents for STAT4 Antibody (10H10O9)

Certificate of Analysis

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Product Specific Notices for STAT4 Antibody (10H10O9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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