STAT4 Antibody (26G2) - BSA Free

Novus Biologicals | Catalog # NBP3-26300

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 26G2 expressed in HEK293

Format

BSA Free
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Product Specifications

Immunogen

A synthesized peptide derived from Human STAT4 [UniProt Q14765]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for STAT4 Antibody (26G2) - BSA Free

STAT4 Antibody (26G2)

Immunohistochemistry: STAT4 Antibody (26G2) [NBP3-26300] -

Immunohistochemistry: STAT4 Antibody (26G2) [NBP3-26300] - Image of STAT4 Antibody (26G2) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
STAT4 Antibody (26G2)

Immunohistochemistry: STAT4 Antibody (26G2) [NBP3-26300] -

Immunohistochemistry: STAT4 Antibody (26G2) [NBP3-26300] - Image of STAT4 Antibody (26G2) diluted at 1:100 and staining in paraffin-embedded human testis tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
STAT4 Antibody (26G2)

Immunocytochemistry/Immunofluorescence: STAT4 Antibody (26G2) [NBP3-26300] -

Immunocytochemistry/Immunofluorescence: STAT4 Antibody (26G2) [NBP3-26300] - Staining of HepG2 cell with STAT4 Antibody (26G2) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 515-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for STAT4 Antibody (26G2) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: STAT4

STAT4 is encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.

Long Name

Signal Transducer and Activator of Transcription 4

Alternate Names

signal transducer and activator of transcription 4, SLEB11

Gene Symbol

STAT4

Additional STAT4 Products

Product Documents for STAT4 Antibody (26G2) - BSA Free

Certificate of Analysis

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Product Specific Notices for STAT4 Antibody (26G2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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