STAT4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89474

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all STAT4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PMHVAVVISNCLREERRILAAANMPVQGPLEKSLQSSSVSERQRNVEHKVAAIKNSVQMTEQDTKYLEDLQDEFDYRYKTIQTMDQSDKNSAMVNQEVLTLQEMLNSLDFKRKEALSKMTQIIHETDL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit STAT4 Antibody - BSA Free (NBP1-89474) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STAT4 Antibody - BSA Free

STAT4 Antibody - BSA Free Immunohistochemistry-Paraffin: STAT4 Antibody - BSA Free [NBP1-89474]

Immunohistochemistry-Paraffin: STAT4 Antibody - BSA Free [NBP1-89474]

Analysis in human testis and skeletal muscle tissues using Anti-STAT4 antibody. Corresponding STAT4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: STAT4 Antibody [NBP1-89474]

Immunohistochemistry-Paraffin: STAT4 Antibody [NBP1-89474]

Immunohistochemistry-Paraffin: STAT4 Antibody [NBP1-89474] - Staining of human testis shows high expression.
Immunohistochemistry-Paraffin: STAT4 Antibody [NBP1-89474]

Immunohistochemistry-Paraffin: STAT4 Antibody [NBP1-89474]

Immunohistochemistry-Paraffin: STAT4 Antibody [NBP1-89474] - Staining of human skeletal muscle shows low expression as expected.
STAT4 Antibody - BSA Free Western Blot: STAT4 Antibody - BSA Free [NBP1-89474]

Western Blot: STAT4 Antibody - BSA Free [NBP1-89474]

Analysis in control (vector only transfected HEK293T lysate) and STAT4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).

Applications for STAT4 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STAT4

STAT4 is encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.

Long Name

Signal Transducer and Activator of Transcription 4

Alternate Names

signal transducer and activator of transcription 4, SLEB11

Gene Symbol

STAT4

Additional STAT4 Products

Product Documents for STAT4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for STAT4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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