Human STAT5A ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-80280

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and other biological fluids (100 ul)

Sensitivity

0.375 ng/mL

Assay Range

0.625-40 ng/mL
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Product Specifications

Assay Type

Sandwich-ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This kit recognizes Human STAT5A in samples. No significant cross-reactivity or interference between Human STAT5A and analogues was observed.

Precision

Intra-Assay Precision (Precision within an assay) CV% 5.72%

Inter-Assay Precision (Precision between assays) CV% 5.04%

Recovery for Human STAT5A ELISA Kit (Colorimetric)

Recovery

88-107%

Linearity

Human STAT5A ELISA Kit (Colorimetric)

Scientific Data Images for Human STAT5A ELISA Kit (Colorimetric)

Human STAT5A ELISA Kit (Colorimetric)

ELISA: Human STAT5A ELISA Kit (Colorimetric) [NBP2-80280] -

ELISA: Human STAT5A ELISA Kit (Colorimetric) [NBP2-80280] - Standard Curve Reference

Kit Contents for Human STAT5A ELISA Kit (Colorimetric)

  • Biotinylated Detection Ab Diluent
  • Concentrated Biotinylated Detection Ab (100X)
  • Concentrated HRP Conjugate (100X)
  • Concentrated Wash Buffer (25X)
  • HRP Conjugate Diluent
  • Micro ELISA Plate (Dismountable)
  • Plate Sealer
  • Product Manual
  • Reference Standard
  • Sample Diluent
  • Stop Solution
  • Substrate Reagent

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: STAT5a

Stat5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor (1). Signal transducer and activator of transcription 5 (Stat5) functions both in signal transduction and activation of transcription. It has been found that cytoplasmic Stat5 is translocated to the nucleus in response to phosphorylation. Stat5 225; tyrosine phosphorylation is activated predominantly by IL2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF andGM-CSF(2). Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. NCoA-1/SRC-1 acts as a coactivator for both the alpha- and beta-isoforms of Stat5 (3).

Long Name

Signal Transducer and Activator of Transcription 5

Alternate Names

MGF, signal transducer and activator of transcription 5A, STAT5

Gene Symbol

STAT5A

UniProt

P42229 (Human)

Additional STAT5a Products

Product Documents for Human STAT5A ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human STAT5A ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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