Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # J2D2

Format

BSA Free
View all STEAP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human STEAP1 (1-70aa) purified from E. coli (NP_036581).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Novus Biologicals Mouse STEAP1 Antibody (J2D2) - BSA Free (NBP1-07094) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for STEAP1 Antibody (J2D2) - BSA Free

Immunocytochemistry/ Immunofluorescence: STEAP1 Antibody (J2D2) - BSA Free [NBP1-07094]

Immunocytochemistry/ Immunofluorescence: STEAP1 Antibody (J2D2) - BSA Free [NBP1-07094]

Immunocytochemistry/Immunofluorescence: STEAP1 Antibody (J2D2) [NBP1-07094] - staining of human LNCap cell colony with monoclonalanti-human STEAP1 antibody (J2D2).
Immunohistochemistry-Paraffin: STEAP1 Antibody (J2D2) - BSA Free [NBP1-07094]

Immunohistochemistry-Paraffin: STEAP1 Antibody (J2D2) - BSA Free [NBP1-07094]

Immunohistochemistry-Paraffin: STEAP1 Antibody (J2D2) [NBP1-07094] - Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB). Human breast cancer tissue (left) and prostate hyperplasia tissue (right).
STEAP1 Antibody (J2D2) - BSA Free

Western Blot: STEAP1 Antibody (J2D2) [NBP1-07094] -

Western Blot: STEAP1 Antibody (J2D2) [NBP1-07094] - The cell lysates (30ug) and tissue lysates (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with NBP1-07094 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: LNcap cell lysate, Lane 2: Mouse Testis Tissue lysate

Applications for STEAP1 Antibody (J2D2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50SDS-PAGE 1:100-1:2000

Western Blot

1:1000-1:2000
Application Notes
For IHC-P: Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB).

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: STEAP1

Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing's sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy. Clone J2D2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human STEAP1 protein.

Long Name

Six Transmembrane Epithelial Antigen of the Prostate 1

Alternate Names

PRSS24

Entrez Gene IDs

26872 (Human)

Gene Symbol

STEAP1

UniProt

NP_036581

Additional STEAP1 Products

Product Documents for STEAP1 Antibody (J2D2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for STEAP1 Antibody (J2D2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers
Redox Enzymes

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for STEAP1 Antibody (J2D2) - BSA Free

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  • Q: Can I received overnight shipping on NBP1-07094 (anti-STEAP1 Mouse IgG2b antibody)?

    A: Unfortunately, NBP1-07094 is currently available in 6 to 8 business days, as is stated on our website. I apologize for the inconvenience. To view the price and availability of our products, please use our website to select your country, which will allow you to view the country specific pricing, availability and technical support information on the product's datasheet.

  • Q: May any of your anti human-STEAP1 Abs be used for flow cytometry, with a secondary anti-mouse antibody? E.g. J2D2 or 4F6-1F3?

    A: Unfortunately, none of our STEAP1 antibodies have been validated in flow cytometry yet. This does not mean they will not work in FLOW, we just cannot guarantee its use in FLOW application because we have not tested them in this application in our lab yet. However, if you would like to try one of these antibodies in FLOW, you would be eligible for our Innovator's Reward, whereby if you share your results with us, we will reward you with a voucher for 100% of the purchase price - see here for more details: Innovator's Reward program. Technically, STEAP1 (J2D2) NBP1-07094 and STEAP1 (4F6-1F3) H00026872-M01 are the two antibodies that have more chances of success because of proven compatibility with IF labeled secondary antibodies as they have already been validated in ICC-IF.

  • Q: Can I received overnight shipping on NBP1-07094 (anti-STEAP1 Mouse IgG2b antibody)?

    A: Unfortunately, NBP1-07094 is currently available in 6 to 8 business days, as is stated on our website. I apologize for the inconvenience. To view the price and availability of our products, please use our website to select your country, which will allow you to view the country specific pricing, availability and technical support information on the product's datasheet.

  • Q: May any of your anti human-STEAP1 Abs be used for flow cytometry, with a secondary anti-mouse antibody? E.g. J2D2 or 4F6-1F3?

    A: Unfortunately, none of our STEAP1 antibodies have been validated in flow cytometry yet. This does not mean they will not work in FLOW, we just cannot guarantee its use in FLOW application because we have not tested them in this application in our lab yet. However, if you would like to try one of these antibodies in FLOW, you would be eligible for our Innovator's Reward, whereby if you share your results with us, we will reward you with a voucher for 100% of the purchase price - see here for more details: Innovator's Reward program. Technically, STEAP1 (J2D2) NBP1-07094 and STEAP1 (4F6-1F3) H00026872-M01 are the two antibodies that have more chances of success because of proven compatibility with IF labeled secondary antibodies as they have already been validated in ICC-IF.

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