STI1 Antibody (4B6) - Azide and BSA Free

Novus Biologicals | Catalog # H00010963-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 4B6

Format

Azide and BSA Free
View all STI1 Primary Antibodies »

Product Specifications

Immunogen

STIP1 (AAH02987.1, 1 a.a. ~ 543 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEQVNELKEKGNKALSVGNIDDALQCYSEAIKLDPHNHVLYSNRSAAYAKKGDYQKAYEDGCKTVDLKPDWGKGYSRKAAALEFLNRFEEAKRTYEEGLKHEANNPQLKEGLQNMEARLAERKFMNPFNMPNLYQKLESDPRTRTLLSDPTYRELIEQLRNKPSDLGTKLQDPRIMTTLSVLLGVDLGSMDEEEEIATPPPPPPPKKETKPEPMEEDLPENKKQALKEKELGNDAYKKKDFDTALKHYDKAKELDPTNMTYITNQAAVYFEKGDYNKCRELCEKAIEVGRENREDYRQIAKAYARIGNSYFKEEKYKDAIHFYNKSLAEHRTPDVLKKCQQAEKILKEQERLAYINPDLALEEKNKGNECFQKGDYPQAMKHYTEAIKRNPKDAKLYSNRAACYTKLLEFQLALKDCEECIQLEPTFIKGYTRKAAALEAMKDYTKAMDVYQKALDLDSSCKEAADGYQRCMMAQYNRHDSPEDVKRRAMADPEVQQIMSDPAMRLILEQMQKDPQALSEHLKNPVIAQKIQKLMDVGLIAIR

Reactivity Notes

Human. Other species not tested.

Specificity

STIP1 - stress-induced-phosphoprotein 1 (Hsp70/Hsp90-organizing protein)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for STI1 Antibody (4B6) - Azide and BSA Free

Western Blot: STI1 Antibody (4B6) [H00010963-M01]

Western Blot: STI1 Antibody (4B6) [H00010963-M01]

Western Blot: STI1 Antibody (4B6) [H00010963-M01] - STIP1 monoclonal antibody (M01), clone 4B6 Analysis of STIP1 expression in HeLa.
Immunocytochemistry/ Immunofluorescence: STI1 Antibody (4B6) [H00010963-M01]

Immunocytochemistry/ Immunofluorescence: STI1 Antibody (4B6) [H00010963-M01]

Immunocytochemistry/Immunofluorescence: STI1 Antibody (4B6) [H00010963-M01] - Analysis of monoclonal antibody to STIP1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: STI1 Antibody (4B6) [H00010963-M01]

Immunohistochemistry-Paraffin: STI1 Antibody (4B6) [H00010963-M01]

Immunohistochemistry-Paraffin: STI1 Antibody (4B6) [H00010963-M01] - Analysis of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human testis. Antibody concentration 0.3 ug/ml.
Western Blot: STI1 Antibody (4B6) [H00010963-M01]

Western Blot: STI1 Antibody (4B6) [H00010963-M01]

Western Blot: STI1 Antibody (4B6) [H00010963-M01] - Analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody (M01), clone 4B6.Lane 1: STIP1 transfected lysate(62.6 KDa).Lane 2: Non-transfected lysate.
Immunoprecipitation: STI1 Antibody (4B6) [H00010963-M01]

Immunoprecipitation: STI1 Antibody (4B6) [H00010963-M01]

Immunoprecipitation: STI1 Antibody (4B6) [H00010963-M01] - Analysis of STIP1 transfected lysate using anti-STIP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STIP1 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: STI1 Antibody (4B6) [H00010963-M01] - Detection limit for recombinant GST tagged STIP1 is approximately 0.1ng/ml as a capture antibody.

Applications for STI1 Antibody (4B6) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: STI1

STIP1 is an adaptor protein that coordinates the functions of HSP70 (see HSPA1A; MIM 140550) and HSP90 (see HSP90AA1; MIM 140571) in protein folding. It is thought to assist in the transfer of proteins from HSP70 to HSP90 by binding both HSP90 and substrate-bound HSP70. STIP1 also stimulates the ATPase activity of HSP70 and inhibits the ATPase activity of HSP90, suggesting that it regulates both the conformations and ATPase cycles of these chaperones (Song and Masison, 2005 (PubMed 16100115)).(supplied by OMIM)

Long Name

Stress-induced Phosphoprotein 1

Alternate Names

HOP, IEF-SSP-3521, NY-REN-11, STI1L, STIP1

Entrez Gene IDs

10963 (Human)

Gene Symbol

STIP1

OMIM

605063 (Human)

UniProt

AAH02987 (Human)

Additional STI1 Products

Product Documents for STI1 Antibody (4B6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for STI1 Antibody (4B6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for STI1 Antibody (4B6) - Azide and BSA Free

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Protocols

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FAQs

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