STING/TMEM173 Antibody (9V9A3)

Novus Biologicals | Catalog # NBP3-57378

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9V9A3
View all STING/TMEM173 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein (or fragment).This information is considered to be commercially sensitive. (Swissprot# Q86WV6)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

Normal Tissues Tested
Pancreas, Lung, Tonsil, Cervix, Placenta, Colon, Kidney, Spleen, Liver, esophagus

Cancer Tissues Tested
Breast cancer, Colon cancer, Thyroid cancer, Hepatocellular Carcinoma, Hepatocholangio carcinoma, Lung adenocarcinoma, Lung squamous carcinoma

Positive Controls
Tonsil, lung, spleen, endothelial cells

Negative Controls
Liver (Cholangiocytes/Hepatocytes), breast

Scientific Data Images for STING/TMEM173 Antibody (9V9A3)

STING/TMEM173 Antibody (9V9A3) Immunocytochemistry/ Immunofluorescence: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunocytochemistry/ Immunofluorescence: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Confocal imaging of THP-1 cells using NBP3-57378 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
STING/TMEM173 Antibody (9V9A3) Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Confocal imaging of paraffin-embedded Human colon cancer tissue using NBP3-57378 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
STING/TMEM173 Antibody (9V9A3) Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Confocal imaging of paraffin-embedded Human tonsil tissue using NBP3-57378 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
STING/TMEM173 Antibody (9V9A3) Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Confocal imaging of paraffin-embedded Rat spleen tissue using NBP3-57378 (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
STING/TMEM173 Antibody (9V9A3) Immunoprecipitation: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunoprecipitation: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunoprecipitation of STING/TMEM173 from 1000 µg extracts of HT-29 cells was performed using 2 ug NBP3-57378. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using NBP3-57378 at a dilution of 1:3000.
STING/TMEM173 Antibody (9V9A3) Western Blot: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Western Blot: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Western blot analysis of various lysates using NBP3-57378 at 1:5000 dilution incubated overnight at 4C. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s.
STING/TMEM173 Antibody (9V9A3) Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using NBP3-57378 at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
STING/TMEM173 Antibody (9V9A3) Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using NBP3-57378 at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
STING/TMEM173 Antibody (9V9A3) Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using NBP3-57378 at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
STING/TMEM173 Antibody (9V9A3) Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry-Paraffin: STING/TMEM173 Antibody (9V9A3) [NBP3-57378]

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using NBP3-57378 at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Applications for STING/TMEM173 Antibody (9V9A3)

Application
Recommended Usage

ELISA

1 ug/mL

Immunocytochemistry/ Immunofluorescence

1:200-1:800

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

0.5ug-4ug antibody for 600ug-1000ug extracts of whole cells

Western Blot

1:2000 - 1:20000
Application Notes
For immunofluorescence on paraffin-embedded tissues, the recommended concentration is 1:200-1:800

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, 0.05% BSA, (pH 7.3)

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: STING/TMEM173

STING (stimulator of interferon genes) is encoded by the TMEM173 gene and is an adaptor molecule involved in the activation of innate immune responses to PAMPS (pathogen-associated molecular patterns) and DAMPS (damage-associated molecular patterns). STING specifically recognizes cytosolic DNA products derived from pathogens (e.g., cytomegalovirus, vaccinia virus, Listeria monocytogenes) or dead cells (1, 2). In the STING pathway, dsDNA derived from pathogens or damaged cells serves as a substrate for the enzyme cGAS (cyclic GMP-AMP synthase) which produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP (3, 4). Under steady-state conditions STING (theoretical molecular weight 42 kDa), a protein localizes to the ER membrane. Upon activation by dsDNA derived second messenger (cGAMP), STING translocates to the Golgi apparatus as a homodimer. Once STING has trafficked to the perinuclear region, it activates TANK binding kinase 1 (TBK1), interferon regulatory factor 3 (IRF3) and NF-kB leading to the production of cytokines (e.g., type I interferon) (2, 4). Mutations in the TMEM173 gene affecting STING expression are associated with the development of the auto-inflammatory disease SAVI (STING-associated vasculopathy with onset in infancy) (2). A novel SAVI dominant mutation in the TMEM173 human gene (V155M) leads to increased localization of STING to the Golgi and perinuclear region, indicative of an activated state (1). Hallmarks of SAVI, a rare inflammatory disease, include severe vasculitis in extremities and lung inflammation (7).

References

1. Patel, S., & Jin, L. (2019). TMEM173 variants and potential importance to human biology and disease. Genes and Immunity. https://doi.org/10.1038/s41435-018-0029-9

2. Jounai, N., Kobiyama, K., Takeshita, F., & Ishii, K. J. (2013). Recognition of damage-associated molecular patterns related to nucleic acids during inflammation and vaccination. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2012.00168

3. Xiao, T. S., & Fitzgerald, K. A. (2013). The cGAS-STING Pathway for DNA Sensing. Molecular Cell. https://doi.org/10.1016/j.molcel.2013.07.004

4. Kato, K., Omura, H., Ishitani, R., & Nureki, O. (2017). Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA. Annual Review of Biochemistry. https://doi.org/10.1146/annurev-biochem-061516-044813

5. Crowl, J. T., Gray, E. E., Pestal, K., Volkman, H. E., & Stetson, D. B. (2017). Intracellular Nucleic Acid Detection in Autoimmunity. Annual Review of Immunology. https://doi.org/10.1146/annurev-immunol-051116-052331

Long Name

Stimulator of Interferon Genes Protein/Transmembrane protein 173

Alternate Names

ERIS, MITA, MPYS, NET23, TMEM173

Gene Symbol

STING1

Additional STING/TMEM173 Products

Product Documents for STING/TMEM173 Antibody (9V9A3)

Certificate of Analysis

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Product Specific Notices for STING/TMEM173 Antibody (9V9A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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