STK16 Antibody (M2) - Azide and BSA Free
Novus Biologicals | Catalog # H00008576-M02
![Western Blot: STK16 Antibody (M2) [H00008576-M02]](https://resources.rndsystems.com/images/products/STK16-Antibody-M2-Western-Blot-H00008576-M02-img0008.jpg "Western Blot: STK16 Antibody (M2) [H00008576-M02]")
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Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # M2
Format
Azide and BSA Free
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Product Specifications
Immunogen
STK16 (AAH02618, 1 a.a. ~ 305 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGHALCVCSRGTVIIDNKRYLFIQKLGEGGFSYVDLVEGLHDGHFYALKRILCHEQQDREEAQREADMHRLFNHPNILRLVAYCLRERGAKHEAWLLLPFFKRGTLWNEIERLKDKGNFLTEDQILWLLLGICRGLEAIHAKGYAHRDLKPTNILLGDEGQPVLMDLGSMNQACIHVEGSRQALTLQDWAAQRCTISYRAPELFSVQSHCVIDERTDVWSLGCVLYAMMFGEGPYDMVFQKGDSVALAVQNQLSIPQSPRHSSALWQLLNSMMTVDPHQRPHIPLLLSQLEALQPPAPGQHTTQI
Specificity
STK16 - serine/threonine kinase 16
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for STK16 Antibody (M2) - Azide and BSA Free
Western Blot: STK16 Antibody (M2) [H00008576-M02]
Western Blot: STK16 Antibody (M2) [H00008576-M02] - STK16 monoclonal antibody (M02), clone M2 Analysis of STK16 expression in A-431.![Immunohistochemistry-Paraffin: STK16 Antibody (M2) [H00008576-M02]](https://resources.rndsystems.com/images/products/STK16-Antibody-M2-Immunohistochemistry-Paraffin-H00008576-M02-img0006.jpg "Immunohistochemistry-Paraffin: STK16 Antibody (M2) [H00008576-M02]")
Immunohistochemistry-Paraffin: STK16 Antibody (M2) [H00008576-M02]
Immunohistochemistry-Paraffin: STK16 Antibody (M2) [H00008576-M02] - Analysis of monoclonal antibody to STK16 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.![Western Blot: STK16 Antibody (M2) [H00008576-M02]](https://resources.rndsystems.com/images/products/STK16-Antibody-M2-Western-Blot-H00008576-M02-img0007.jpg "Western Blot: STK16 Antibody (M2) [H00008576-M02]")
Western Blot: STK16 Antibody (M2) [H00008576-M02]
Western Blot: STK16 Antibody (M2) [H00008576-M02] - Analysis of STK16 over-expressed 293 cell line, cotransfected with STK16 Validated Chimera RNAi ( Cat # H00008576-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STK16 monoclonal antibody (M02), clone M2 (Cat # H00008576-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: STK16 Antibody (M2) [H00008576-M02]](https://resources.rndsystems.com/images/products/STK16-Antibody-M2-Western-Blot-H00008576-M02-img0009.jpg "Western Blot: STK16 Antibody (M2) [H00008576-M02]")
Western Blot: STK16 Antibody (M2) [H00008576-M02]
Western Blot: STK16 Antibody (M2) [H00008576-M02] - Analysis of STK16 expression in transfected 293T cell line by STK16 monoclonal antibody (M02), clone M2.Lane 1: STK16 transfected lysate(34.7 KDa).Lane 2: Non-transfected lysate.Applications for STK16 Antibody (M2) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P, RNAi Validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: STK16
Long Name
Serine/threonine-protein kinase 16
Alternate Names
hPSK, MPSK, MPSK1, PKL12, TSF-1, TSF1
Entrez Gene IDs
8576 (Human)
Gene Symbol
STK16
OMIM
604719 (Human)
UniProt
Additional STK16 Products
Product Documents for STK16 Antibody (M2) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for STK16 Antibody (M2) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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