STK38 Antibody (2F6)

Novus Biologicals | Catalog # H00011329-M11

Novus Biologicals
Discontinued Product
H00011329-M11 has been discontinued. View all NDR1/STK38 products.

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F6
View all NDR1/STK38 Primary Antibodies »

Product Specifications

Immunogen

STK38 (AAH12085, 365 a.a. ~ 465 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EHRIGAPGVEEIKSNSFFEGVDWEHIRERPAAISIEIKSIDDTSNFDEFPESDILKPTVATSNHPETDYKNKDWVFINYTYKRFEGLTARGAIPSYMKAAK

Specificity

STK38 (2F6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for STK38 Antibody (2F6)

Western Blot: STK38 Antibody (2F6) [H00011329-M11]

Western Blot: STK38 Antibody (2F6) [H00011329-M11]

Western Blot: STK38 Antibody (2F6) [H00011329-M11] - Analysis of STK38 expression in Hela S3 NE (Cat # L013V3).
Immunocytochemistry/ Immunofluorescence: STK38 Antibody (2F6) [H00011329-M11]

Immunocytochemistry/ Immunofluorescence: STK38 Antibody (2F6) [H00011329-M11]

Immunocytochemistry/Immunofluorescence: STK38 Antibody (2F6) [H00011329-M11] - Analysis of monoclonal antibody to STK38 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: STK38 Antibody (2F6) [H00011329-M11]

Immunohistochemistry-Paraffin: STK38 Antibody (2F6) [H00011329-M11]

Immunohistochemistry-Paraffin: STK38 Antibody (2F6) [H00011329-M11] - Analysis of monoclonal antibody to STK38 on formalin-fixed paraffin-embedded human colon. Antibody concentration 2 ug/ml
Western Blot: STK38 Antibody (2F6) [H00011329-M11]

Western Blot: STK38 Antibody (2F6) [H00011329-M11]

Western Blot: STK38 Antibody (2F6) [H00011329-M11] - Analysis of STK38 expression in human kidney.
ELISA: STK38 Antibody (2F6) [H00011329-M11]

ELISA: STK38 Antibody (2F6) [H00011329-M11]

ELISA: STK38 Antibody (2F6) [H00011329-M11] - Detection limit for recombinant GST tagged STK38 is approximately 0.3ng/ml as a capture antibody.

Applications for STK38 Antibody (2F6)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NDR1/STK38

STK38 belongs to the NDR family of serine/threonine protein kinases. NDR kinases require the phosphorylation of conserved Ser/Thr residues for activation. NDR family members have two unique stretches of primary sequence: an N-terminal regulatory (NTR) domain and an insert of several residues between subdomains VII and VIII of the kinase domain. The kinase domain insert functions as an auto-inhibitory sequence (AIS), while binding of the co-activator MOB (Mps-one binder) proteins to the NTR domain releases NDR kinases from inhibition of autophosphorylation. STK38 negatively regulates the activation of MEKK1/2 by direct interaction with the catalytic domain of MEKK1/2. The negative regulation of MEKK1/2 is not due to its phosphorylation by STK38.

Long Name

Nuclear Dbf2-related Kinase 1

Alternate Names

STK38

Entrez Gene IDs

11329 (Human)

Gene Symbol

STK38

OMIM

606964 (Human)

UniProt

AAH12085 (Human)

Additional NDR1/STK38 Products

Product Documents for STK38 Antibody (2F6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for STK38 Antibody (2F6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for STK38 Antibody (2F6)

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Protocols

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