SUMO Conjugation Negative Control Peptide Substrate, Biotin
SUMO Conjugation Negative Control Peptide Substrate, Biotin Summary
Specifications
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Product Datasheets
Background: SUMO
This biotinylated peptide serves as a negative control and contains a scrambled version of the consensus motif sequence (SP-300). It will not be conjugated to SUMO1, SUMO2 or SUMO3 proteins in the presence of the E1 activating (SAE1/SAE2) and Ubc9 conjugating enzymes. The biotin group allows for sensitive and convenient detection using avidin-linked reagents. SUMO modification of proteins occurs on lysine residues generally found within a short consensus sequence containing the psi ΚXE motif. This motif consists of psi which represents a large hydrophobic amino acid (isoleucine, leucine or valine), K is the lysine that becomes modified, X is any residue and E is glutamic acid. The glutamic acid is the most highly conserved position other than the lysine.
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