SUMO Conjugation Peptide Substrate, Biotin

Catalog # Availability Size / Price Qty
SP-300-020
Product Details
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SUMO Conjugation Peptide Substrate, Biotin Summary

This biotinylated peptide contains a Sumoylation consensus sequence and can be covalently conjugated to SUMO1, SUMO2 and SUMO3 proteins in the presence of the E1 activating (SAE1/SAE2) and Ubc9 (UBE2I) conjugating enzymes. The biotin group allows for convenient and sensitive detection with avidin-linked reagents.SUMO modification of proteins occurs on lysine residues generally found within a short consensus sequence containing the psi ΚXE motif. This motif consists of psi which represents a large hydrophobic amino acid (isoleucine, leucine or valine), K is the lysine that becomes modified, X is any residue and E is glutamic acid. The glutamic acid is the most highly conserved position other than the lysine.

Specifications

Source
Synthetic
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Applications
Enzyme Activity
Species
Multi-Species

Product Datasheets

Background: SUMO

Long Name
Small Ubiquitin-like Modifiers
Alternate Names
SUMO

Reagents Provided

FAQs

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Supplemental Ubiquitin Reagents

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