SUN1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87395

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SUN1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QGNFFSFLPVLNWASMHRTQRVDDPQDVFKPTTSRLKQPLQGDSEAFPWHWMSGVEQQVASLSGQCHHHGENLRELTTLLQKLQARVDQMEGGAAGPSASVRDAVGQPPRETDFMAFHQEHEVRMSHLEDILGKLR

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 26356418).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SUN1 Antibody - BSA Free

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Analysis in human skin and bone marrow tissues. Corresponding SUN1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human fallopian tube, kidney, skin and testis using Anti-SUN1 antibody NBP1-87395 (A) shows similar protein distribution across tissues to independent antibody NBP1-87396 (B).
Western Blot: SUN1 Antibody [NBP1-87395]

Western Blot: SUN1 Antibody [NBP1-87395]

Western Blot: SUN1 Antibody [NBP1-87395] - Analysis using Anti-SUN1 antibody NBP1-87395 (A) shows similar pattern to independent antibody NBP1-87396 (B).
Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87395]

Immunocytochemistry/ Immunofluorescence: SUN1 Antibody [NBP1-87395]

Immunocytochemistry/Immunofluorescence: SUN1 Antibody [NBP1-87395] - Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane & vesicles.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human testis shows strong positivity in nuclear membrane in Leydig cells.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human bone marrow shows low positivity in hematopoietic cells as expected.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human Fallopian tube shows moderate positivity in nuclear membrane in glandular cells.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human kidney shows strong positivity in nuclear membrane in cells in glomeruli.
Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395]

Immunohistochemistry-Paraffin: SUN1 Antibody [NBP1-87395] - Staining of human skin shows strong positivity in nuclear membrane in squamous epithelial cells.

Applications for SUN1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF reported in scientific literature (PMID: 26356418). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SUN1

This gene is a member of the unc-84 homolog family and encodes a nuclear nuclear envelope protein with an Unc84 (SUN) domain. The protein is involved in nuclear anchorage and migration. Several alternatively spliced transcript variants of this gene have been described; however, the full-length nature of some of these variants has not been determined.

Alternate Names

KIAA0810UNC84AFLJ12407, MGC176649, Protein unc-84 homolog A, Sad1 and UNC84 domain containing 1, Sad1 unc-84 domain protein 1, Sad1/unc-84 protein-like 1, SUN domain-containing protein 1, unc-84 homolog A, unc-84 homolog A (C. elegans)

Gene Symbol

SUN1

Additional SUN1 Products

Product Documents for SUN1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SUN1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for SUN1 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for SUN1 Antibody - BSA Free

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  • Q: In the third picture on the website, what is the red and what is the green?

    A: SUN1 is a nuclear protein, so the green is SUN1 and the red is actin staining to see the cell shape and structure.

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