Suppressor of Fused Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35342

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Suppressor of Fused Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 325-484 of human Suppressor of Fused (NP_057253.2).

Sequence:
PPINPQRQNGLAHDRAPSRKDSLESDSSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Suppressor of Fused Antibody - BSA Free

Suppressor of Fused Antibody

Immunocytochemistry/ Immunofluorescence: Suppressor of Fused Antibody [NBP3-35342] -

Immunocytochemistry/ Immunofluorescence: Suppressor of Fused Antibody [NBP3-35342] - Immunofluorescence analysis of MCF-7 cells using Suppressor of Fused Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
Suppressor of Fused Antibody

Immunohistochemistry: Suppressor of Fused Antibody [NBP3-35342] -

Immunohistochemistry: Suppressor of Fused Antibody [NBP3-35342] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using Suppressor of Fused Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Suppressor of Fused Antibody

Immunohistochemistry: Suppressor of Fused Antibody [NBP3-35342] -

Immunohistochemistry: Suppressor of Fused Antibody [NBP3-35342] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Suppressor of Fused Rabbit pAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Suppressor of Fused Antibody

Western Blot: Suppressor of Fused Antibody [NBP3-35342] -

Western Blot: Suppressor of Fused Antibody [NBP3-35342] - Western Blot analysis of lysates from Mouse testis using Suppressor of Fused Rabbit pAbat 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

Applications for Suppressor of Fused Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Suppressor of Fused

The Hedgehog (Hh) signaling pathway has conserved roles in development of species ranging from Drosophila to humans. Human supressor of fused HSu(Fu) acts as a negative regulator in the hedgehog signaling (SHH) pathway by down-regulating GLI1-mediated transactivation of target genes (1). HSu(fu) was found to repress activity of the zinc-finger transcription factor Gli, which mediates Hedgehog signaling in vertebrates, and to physically interact with Gli, Gli2 and Gli3 (2). The sonic hedgehog signaling pathway directs the embryonic development of diverse organisms and is disrupted in a variety of malignancies. Several of mutations encode truncated proteins that are unable to export the GLI transcription factor from nucleus to cytoplasm, resulting in the activation of SHH signaling. SUFU is a newly identified tumor-suppressor gene that predisposes individuals to medulloblastoma by modulating the SHH signaling pathway through a newly identified mechanism (3).

Alternate Names

SUFUHSUFUXLPRO1280, suppressor of fused homolog, suppressor of fused homolog (Drosophila)

Gene Symbol

SUFU

Additional Suppressor of Fused Products

Product Documents for Suppressor of Fused Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Suppressor of Fused Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for Suppressor of Fused Antibody - BSA Free

There are currently no reviews for this product. Be the first to review Suppressor of Fused Antibody - BSA Free and earn rewards!

Have you used Suppressor of Fused Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies